Wandall A, Svendsen A
Chromosoma. 1985;92(4):254-64. doi: 10.1007/BF00329808.
Aedes aegypti spermatocytes were reconstructed from electron micrographs. The species has tight somatic pairing of the chromosomes, and there are therefore no classical leptotene and zygotene stages, but rather a gradual transition from somatic pairing to meiotic pairing (= pachytene). The term "prepachytene" has been used for the transitory stage. The first visible sign of impending meiosis was a reorganization of the chromatin, which resulted in the formation of spaces (synaptic spaces) in the chromatin, about the width of the synaptonemal complexes (SCs). Diffuse material, possibly precursor material for the SC, was present in the spaces. Later short pieces of complex were formed throughout the nucleus. Late prepachytene, pachytene, and diplotene complexes were reconstructed. Each chromosome occupied a separate region of the nucleus. The complexes became progressively shorter from prepachytene (maximum complement length 289 micron) to diplotene (175 micron). The thickness of the SCs increased from prepachytene to pachytene and probably decreased again during diplotene. At the beginning of diplotene the lateral elements (LEs) separated, and the single LEs became two to three times thicker than the LEs of the SC. The centromeres were at all stages attached to the nuclear membrane, whereas the telomeres were free in the nucleoplasm during pachytene and diplotene. A heterochromatic marker was present on chromosome 1 near the sex determining locus, and a diffuse marker on chromosome 3 near the nucleolus organizer region. After breakdown of the complexes, polycomplexes were present in the nucleus.
埃及伊蚊精母细胞是根据电子显微镜照片重建的。该物种的染色体在体细胞中有紧密配对，因此不存在经典的细线期和偶线期，而是从体细胞配对到减数分裂配对（=粗线期）的逐渐转变。“前粗线期”一词用于这个过渡阶段。即将进行减数分裂的第一个可见迹象是染色质的重新组织，这导致染色质中形成了间隙（突触间隙），其宽度与联会复合体（SCs）相当。间隙中存在弥散物质，可能是SCs的前体物质。后来，整个细胞核中形成了短片段的复合体。重建了前粗线期末期、粗线期和双线期的复合体。每条染色体占据细胞核的一个单独区域。复合体从前粗线期（最大全长289微米）到双线期（175微米）逐渐变短。SCs的厚度从前粗线期到粗线期增加，在双线期可能再次减小。在双线期开始时，侧生元件（LEs）分离，单个LEs比SCs的LEs厚两到三倍。着丝粒在所有阶段都附着在核膜上，而端粒在粗线期和双线期在核质中是游离的。在靠近性别决定位点的1号染色体上有一个异染色质标记，在靠近核仁组织区的3号染色体上有一个弥散标记。复合体解体后，细胞核中存在多复合体。
