El-Maradny Yousra A, Abu-Serie Marwa M, Hashish Mona H, Selim Heba S, Saleh Abdulrahman M, El-Fakharany Esmail M
Protein Research Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg AL-Arab, Alexandria, 21934, Egypt.
Department of Medical Biotechnology, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological Applications (SRTA-City), New Borg AL-Arab, Alexandria, Egypt.
Sci Rep. 2025 Jul 31;15(1):27893. doi: 10.1038/s41598-025-09967-z.
Viruses are responsible for numerous serious outbreaks and pandemics worldwide. In this context, lectins, which are carbohydrate-binding proteins, have garnered attention due to their antiviral properties against RNA and DNA viruses. The antiviral potential of the purified and well-characterized lectin from Pleurotus ostreatus (POL) was assessed against various viruses. POL showed potent antiviral activity against HCV with IC values of 68.75 nM and 52.13 nM for determining the blocking and neutralizing infectivity, respectively. POL exhibited IC values of 42.75 and 14.88 nM against HBV through treatment and blocking mechanisms, respectively. Notably, POL demonstrated a 58.80% binding capacity to cell-surface CD81, while the IC values for targeting the scavenger receptor class B-type I (SR-B1), HCV-NS3/4A protease inhibition, and anti-HBV polymerase activity were established at 10.08, 10.98, and 4.22 nM, respectively. The outcomes of this investigation offer crucial insights into the mechanisms through which POL effectively inhibits infection caused by different viruses. These findings have the potential to inform the development of antiviral strategies and therapeutic interventions.
病毒是全球众多严重疫情和大流行的罪魁祸首。在此背景下，凝集素作为一类碳水化合物结合蛋白，因其对RNA和DNA病毒的抗病毒特性而备受关注。对平菇纯化且特性明确的凝集素（POL）针对多种病毒的抗病毒潜力进行了评估。POL对丙肝病毒（HCV）显示出强大的抗病毒活性，在测定阻断和中和感染性时，其IC值分别为68.75 nM和52.13 nM。通过治疗和阻断机制，POL对乙肝病毒（HBV）的IC值分别为42.75 nM和14.88 nM。值得注意的是，POL对细胞表面CD81的结合能力为58.80%，而靶向B1型清道夫受体（SR-B1）、抑制HCV-NS3/4A蛋白酶以及抗HBV聚合酶活性的IC值分别为10.08 nM、10.98 nM和4.22 nM。这项研究结果为POL有效抑制不同病毒感染的机制提供了关键见解。这些发现有可能为抗病毒策略和治疗干预的开发提供参考。
