通过RANKL诱导破骨细胞生成机制，结扎诱导性牙周炎后正畸牙齿移动对牙周组织的影响：一项动物研究。

Effects of orthodontic tooth movement on periodontal tissues after ligature-induced periodontitis through the mechanism of RANKL-induced osteoclastogenesis: an animal study.

作者信息

Feyizoglu Buse Basak, Aysesek Batuhan Hazar, Kaya Suheyla, Baser Ulku, Olgac Vakur, Isik Aysen Gulden

机构信息

Faculty of Dentistry, Department of Periodontology, Istanbul University, Zip Code, Vezneciler Klinigi, Kalenderhane Mahallesi Vezneciler Caddesi No:2, 34116, Kalenderhane , Turkey.

Faculty of Dentistry, Department of Periodontology, Istanbul University Cerrahpasa, Istanbul, Turkey.

出版信息

BMC Oral Health. 2025 Jul 30;25(1):1278. doi: 10.1186/s12903-025-06627-6.

DOI:10.1186/s12903-025-06627-6

PMID:40739631

Abstract

BACKGROUND

In recent years, there has been a growing interest in adult orthodontics due to increasing aesthetic demands and advancements in treatment modalities. As more adults seek orthodontic treatment, clinicians frequently encounter patients with pre-existing periodontal conditions, highlighting the need to understand the effects of orthodontic tooth movement (OTM) on periodontal tissues. The aim of this study was to evaluate the response of periodontal tissues to OTM, in healthy conditions and after 14 days of ligature-induced periodontitis, by assessing periodontal ligament (PDL) width and the expression of receptor activator of nuclear factor-κB (RANK)-RANK ligand (RANKL)-osteoprotegerin (OPG) in a rat model of experimental periodontitis and orthodontic tooth movement.

METHODS

Experimental periodontitis was induced by ligating the left maxillary second molars (experimental side) of 36 Wistar rats. The maxillary right sides (control sides) were left unligated as positive controls. After 14 days, the ligatures were removed, and orthodontic appliances for mesialization were placed on three maxillary molars combined in one unit and activated. The rats were sacrificed post-activation on days 1, 7, and 14, with 12 rats per sacrifice day. The amount of OTM was measured and PDL widths were assessed histopathologically. RANK-RANKL-OPG expression was assesed immunohistochemically.

RESULTS

No statistically significant difference was found between the experimental and control sides regarding OTM and PDL width measurements and RANK-RANKL expressions. OPG expression levels were significantly higher on the control sides on day 14 in pressure areas (P < 0.05).

CONCLUSION

Our data indicate that OTM causes similar changes in bone metabolism activity and PDL width in periodontally compromised tissues, compared to healthy controls. These results underscore the clinical relevance of orthodontic treatment as a safe option for patients with a history of periodontitis.

摘要

背景

近年来，由于美学需求的增加和治疗方式的进步，成人正畸越来越受到关注。随着越来越多的成年人寻求正畸治疗，临床医生经常遇到患有牙周疾病的患者，这凸显了了解正畸牙齿移动（OTM）对牙周组织影响的必要性。本研究的目的是通过评估实验性牙周炎和正畸牙齿移动大鼠模型中牙周膜（PDL）宽度以及核因子κB受体激活剂（RANK）-RANK配体（RANKL）-骨保护素（OPG）的表达，来评价健康状态下以及结扎诱导牙周炎14天后牙周组织对OTM的反应。

方法

通过结扎36只Wistar大鼠的左上颌第二磨牙（实验侧）诱导实验性牙周炎。右上颌（对照侧）不结扎作为阳性对照。14天后，拆除结扎线，在三个联合为一个单元的上颌磨牙上放置近中移动的正畸矫治器并激活。在激活后第1、7和14天处死大鼠，每个处死日处死12只大鼠。测量OTM量并通过组织病理学评估PDL宽度。通过免疫组织化学评估RANK-RANKL-OPG表达。