Nolan Christine T, Campbell Ian, Farrell-Sherman Anna, Briones Ortiz Bryan A, Yang Chansie, Naish Kerry A, Di Stilio Verónica S, Kaldy James E, Donoghue Cinde, Ruesink Jennifer L, Imaizumi Takato
Department of Biology, University of Washington, Seattle, WA 98195, USA.
Vaccine and Infectious Disease Division, Fred Hutch Cancer Center, Seattle, WA 98109, USA.
iScience. 2025 Jul 8;28(8):113082. doi: 10.1016/j.isci.2025.113082. eCollection 2025 Aug 15.
Florigen and antiflorigen genes within the phosphatidylethanolamine-binding protein (PEBP) family regulate flowering in angiosperms. In eelgrass (), an estuarine foundation species, flowering, and seed production are crucial for population resilience. Yet, the molecular mechanism underpinning flowering remains unknown. Among 13 genes in (), we showed that four genes (, , , and ) altered flowering time when overexpressed in . We analyzed gene expression in different tissues and throughout the growth season from perennial and annual populations in Willapa Bay and Yaquina Bay, USA. Across six sites exhibiting different degrees of population genetic structure, and were expressed in leaves of vegetative and reproductive shoots and in rhizomes of reproductive shoots. was solely expressed in leaves of vegetative shoots, while levels increased after flowering shoots developed. Our results suggest that and may promote flowering, while may inhibit flowering in eelgrass.
磷脂酰乙醇胺结合蛋白（PEBP）家族中的成花素和抗成花素基因调控被子植物的开花过程。在河口基础物种大叶藻中，开花和种子生产对于种群恢复力至关重要。然而，开花背后的分子机制仍不清楚。在大叶藻的13个基因中，我们发现四个基因（、、和）在大叶藻中过表达时会改变开花时间。我们分析了美国威拉帕湾和亚奎纳湾多年生和一年生种群在不同组织以及整个生长季节的基因表达情况。在六个呈现不同程度种群遗传结构的地点，和在营养枝和生殖枝的叶片以及生殖枝的根茎中表达。仅在营养枝的叶片中表达，而在开花枝发育后水平升高。我们的结果表明，和可能促进大叶藻开花，而可能抑制大叶藻开花。
