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扩展pH值转移技术用于从鱼头中顺序提取完整蛋白质和硫酸化多糖：一种新型生物炼制方法。

Expanding the pH-Shift Technique for Sequential Extraction of Intact Proteins and Sulfated Polysaccharide From Fish Heads: A Novel Biorefinery Approach.

作者信息

Naghdi Shahab, Rezaei Masoud, Tabarsa Mehdi, Abdollahi Mehdi

机构信息

Seafood Processing Department, Marine Sciences Faculty Tarbiat Modares University Noor Iran.

Department of Biology and Biological Engineering-Food and Nutrition Science Chalmers University of Technology Gothenburg Sweden.

出版信息

Food Sci Nutr. 2025 Jul 30;13(8):e70673. doi: 10.1002/fsn3.70673. eCollection 2025 Aug.

DOI:10.1002/fsn3.70673
PMID:40741093
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12310292/
Abstract

The pH-shift technology was successfully expanded for the sequential recovery of proteins and sulfated polysaccharides (SPs) from rainbow trout heads. Adjusting the pH of the remaining process water after protein recovery at their isoelectric point to 8 enabled precipitation and recovery of SPs with the aid of ethanol at both alkaline and acid process versions and named SP-11.5 and SP-2.5, respectively. The mass yield of SPs recovered using the alkaline process version was 3.25%, nearly double that of SP (1.75%) from the acid version. SP-11.5 contained higher levels of carbohydrates (61.22%), proteins (13.29%), and sulfates (12.13%) compared to SP-2.5. FTIR, DSC, and XRD analyses showed no significant differences in the structural properties of the recovered SPs as a function of the pH-shift process version. However, SP-11.5 exhibited better antioxidant activity in DPPH, ABTS, and metal chelating tests and superior antibacterial properties against and than SP-2.5. This study suggests that the pH-shift process can be effectively extended for sequential extraction of both protein isolates and SPs from fish by-products for a multiple product biorefinery where the alkaline version outperformed.

摘要

pH 转变技术已成功扩展，用于从虹鳟鱼头中依次回收蛋白质和硫酸化多糖（SPs）。在蛋白质回收后，将剩余工艺用水的pH值在其等电点调节至8，在碱性和酸性工艺版本中借助乙醇均可实现SPs的沉淀和回收，分别命名为SP-11.5和SP-2.5。使用碱性工艺版本回收的SPs的质量产率为3.25%，几乎是酸性版本中SP（1.75%）的两倍。与SP-2.5相比，SP-11.5含有更高水平的碳水化合物（61.22%）、蛋白质（13.29%）和硫酸盐（12.13%）。傅里叶变换红外光谱（FTIR）、差示扫描量热法（DSC）和X射线衍射（XRD）分析表明，回收的SPs的结构性质不会因pH转变工艺版本而有显著差异。然而，在1,1-二苯基-2-三硝基苯肼（DPPH）、2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）和金属螯合试验中，SP-11.5表现出更好的抗氧化活性，并且对[具体菌种1]和[具体菌种2]的抗菌性能优于SP-2.5。本研究表明pH转变工艺可有效地扩展用于从鱼副产物中依次提取蛋白质分离物和SPs，以实现多产品生物炼制，其中碱性版本表现更优。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8848/12310292/4d2a42fd9533/FSN3-13-e70673-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8848/12310292/d2b2e128b21a/FSN3-13-e70673-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8848/12310292/955862f1da17/FSN3-13-e70673-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8848/12310292/15151d7cb39b/FSN3-13-e70673-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8848/12310292/84548e19ba8c/FSN3-13-e70673-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8848/12310292/7bd708dd2419/FSN3-13-e70673-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8848/12310292/4d2a42fd9533/FSN3-13-e70673-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8848/12310292/d2b2e128b21a/FSN3-13-e70673-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8848/12310292/955862f1da17/FSN3-13-e70673-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8848/12310292/15151d7cb39b/FSN3-13-e70673-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8848/12310292/84548e19ba8c/FSN3-13-e70673-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8848/12310292/7bd708dd2419/FSN3-13-e70673-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8848/12310292/4d2a42fd9533/FSN3-13-e70673-g006.jpg

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