Song Chang, Pang Yu-Yan, Lu Shang-Yi, Li Bin, Li Dong-Ming, He Rong-Quan, Qin Di-Yuan, Li Shi-De, Qv Ning, Chen Yan-Mei, Chen Gang, He Juan, Jiang Xiao-Bo
Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Zhuang Autonomous Region, China.
Department of Hepatological and Gland Surgery, The Seventh Affiliated Hospital of Guangxi Medical University, Wuzhou 543000, Guangxi Zhuang Autonomous Region, China.
World J Clin Oncol. 2025 Jul 24;16(7):107109. doi: 10.5306/wjco.v16.i7.107109.
Although thymopoietin (TMPO) has been elucidated to be overexpressed in cancers, its underlying mechanisms are not yet fully understood.
To investigate the expression and clinical significance of TMPO in papillary thyroid carcinoma (PTC).
Databases such as Gene Expression Omnibus, The Cancer Genome Atlas Program-Genotype-Tissue Expression, The Human Protein Atlas (THPA), and tissue microarrays were screened. Immunohistochemical staining scores and standardized mean difference were used to calculate expression levels, and summary receiver operating characteristic curves were plotted to evaluate diagnostic performance. A Gene Set Enrichment Analysis enrichment analysis was conducted to identify TMPO-related signaling pathways. A protein interaction network was constructed to identify hub genes. The impact of TMPO on PTC cell proliferation and the effects of its knockout were analyzed using clustered regularly interspaced short palindromic repeats (CRISPR) knockout screening and the Cancer Cell Line Encyclopedia database.
The TMPO protein was significantly overexpressed in PTC tissues, primarily localized in the cytoplasm and nuclear membrane. The mRNA level analysis showed mild overexpression of TMPO in PTC tissues, with a certain discriminatory value (area under the curve = 0.66). TMPO may promote cancer through involvement in cell adhesion, focal adhesion, leukocyte migration, and multiple cancer-related signaling pathways. Additionally, gene knockout experiments confirmed that TMPO knockout significantly inhibited the proliferation of PTC cell lines, indicating its important role in tumor growth.
TMPO is overexpressed in PTC and may serve as a therapeutic target and molecular biomarker for PTC.
尽管已阐明胸腺opoietin（TMPO）在癌症中过表达，但其潜在机制尚未完全了解。
研究TMPO在甲状腺乳头状癌（PTC）中的表达及临床意义。
筛选基因表达综合数据库、癌症基因组图谱计划-基因型-组织表达数据库、人类蛋白质图谱（THPA）和组织芯片等。采用免疫组织化学染色评分和标准化均值差计算表达水平，并绘制汇总的受试者工作特征曲线评估诊断性能。进行基因集富集分析以鉴定与TMPO相关的信号通路。构建蛋白质相互作用网络以鉴定枢纽基因。使用成簇规律间隔短回文重复序列（CRISPR）敲除筛选和癌症细胞系百科全书数据库分析TMPO对PTC细胞增殖的影响及其敲除效果。
TMPO蛋白在PTC组织中显著过表达，主要定位于细胞质和核膜。mRNA水平分析显示TMPO在PTC组织中轻度过表达，具有一定的鉴别价值（曲线下面积=0.66）。TMPO可能通过参与细胞黏附、黏着斑、白细胞迁移和多种癌症相关信号通路促进癌症发生。此外，基因敲除实验证实TMPO敲除显著抑制PTC细胞系的增殖，表明其在肿瘤生长中起重要作用。
TMPO在PTC中过表达，可能作为PTC的治疗靶点和分子生物标志物。
