Phytochemicals profile, cellular viabillity and platelet activity of Linn. leaf extract: ex vivo .

OBJECTIVE

to investigates the phytochemical content of Linn leaf extract (HrsL), its toxicity through effects on cell viability, and its impact on platelet activity.

METHODS

The cytotoxicity of the leaf extract was evaluated using fibroblast cells, with various concentrations of the extract. The platelet activity of the HrsL, was assessed by treating blood and analysing the concentration of P-selectin and platelet aggregation with the agonists epinephrine, ADP, and collagen, compared to blood without extract treatment as a control.

RESULTS

The leaf extract contains tannins, polyphenols, alkaloids, and saponins. HrsL was non-toxic at concentrations of 7.8 mg/mL, 15.6 mg/mL, 31.2 mg/mL, 62.5 mg/mL, 125 mg/mL, and 250 mg/mL after 24-h exposure, 7.8 mg/mL, 15.6 mg/mL, 31.2 mg/mL, 62.5 mg/mL, 125 mg/mL, and 250 mg/mL after 48-h exposure, and 15.6 mg/mL and 62.5 mg/mL after 72-h exposure. The effect on platelet activity at 250 mg/mL and 125 mg/mL concentrations of the extract showed a significant increase in P-selectin concentration. Platelet aggregation with the epinephrine agonist did not show significant differences at 7.8 mg/mL, while higher concentrations showed significantly lower aggregation. With the ADP and collagen agonists, no significant differences were observed at concentrations of 7.8 mg/mL, 15.6 mg/mL, 31.2 mg/mL, and 62 mg/mL, whereas concentrations above 62.5 mg/mL showed significantly lower platelet aggregation.

CONCLUSION

HrsL non-toxic at low concentrations and increases platelet P-selectin, It does not decrease platelet aggregation with the epinephrine agonist at 7.8 mg/mL, but it reduces aggregation at concentrations above 7.8 mg/mL. It does not decrease platelet aggregation with the ADP and collagen agonists at concentrations less than or equal to 62.5 mg/mL, but reduces aggregation at concentrations above 62.5 mg/mL.