Peluso Valentina, De Santis Roberto, Gloria Antonio, Castagliuolo Giusy, Zanfardino Anna, Varcamonti Mario, Russo Teresa
Institute of Polymers, Composites and Biomaterials, National Research Council of Italy, 80125 Naples, Italy.
Department of Industrial Engineering, University of Naples Federico II, 80125 Naples, Italy.
ACS Appl Bio Mater. 2025 Aug 18;8(8):6817-6829. doi: 10.1021/acsabm.5c00561. Epub 2025 Jul 31.
: Nonconventional fabrication technologies (, additive manufacturing and 3D bioprinting) represent a challenging approach to the design of 3D scaffolds as extracellular matrix analogues with appropriate properties for supporting cell behavior over time. : A strategy to develop 3D additive manufactured hybrid scaffolds with dual porosity and tailored morphological and mechanical/functional features was proposed the combination of synthetic (poly-ε-caprolactone) and natural (chitosan) polymers. Design of 3D additive manufactured hybrid scaffolds, morphological analysis, swelling and degradation measurements, mechanical measurements, antimicrobial assays against both oral cavity-specific and nonoral bacteria, and biological assays using periodontal ligament stem cells (PDLSCs) or human osteosarcoma cells (MG63) were carried out. : The inclusion of the chitosan network improves the dimensional stability of the structure as well as the cell retention effect, ensuring antimicrobial activity. : The current study represents a first step for future complex works with the aim of studying the effect of the inclusion of chitosan network in a 3D porous multifunctional structure obtained additive manufacturing technologies, also taking into account the possibility of modulating the mechanical behavior, adopting two different swelling and degradation rates in order to tune drug/protein/gene delivery over time, and thus tailoring the tissue regeneration process and the health of the oral microbiota.
非传统制造技术（如增材制造和3D生物打印）是一种具有挑战性的方法，用于设计作为细胞外基质类似物的3D支架，这些支架具有适当的特性，能够长期支持细胞行为。提出了一种开发具有双孔隙率以及定制形态和机械/功能特性的3D增材制造混合支架的策略，即合成聚合物（聚ε-己内酯）和天然聚合物（壳聚糖）的组合。开展了3D增材制造混合支架的设计、形态分析、肿胀和降解测量、力学测量、针对口腔特异性细菌和非口腔细菌的抗菌测定，以及使用牙周膜干细胞（PDLSCs）或人骨肉瘤细胞（MG63）的生物学测定。壳聚糖网络的加入提高了结构的尺寸稳定性以及细胞保留效果，确保了抗菌活性。当前的研究是未来复杂工作的第一步，旨在研究壳聚糖网络加入通过增材制造技术获得的3D多孔多功能结构中的效果，同时考虑调节机械行为的可能性，采用两种不同的肿胀和降解速率来随时间调整药物/蛋白质/基因递送，从而定制组织再生过程和口腔微生物群的健康状况。
