Zaslavskaia P L, Makarevich V G, Slugina M D
Mikrobiologiia. 1977 Mar-Apr;46(2):283-7.
Actinomyces aureofaciens producing tetracycline and its inactive proactinomyces-like variant were studied by electron microscopy during their growth under conditions of regulated and non-regulated fermentation. The ultrastructure of the active variant changed: the cell wall and ribosomes underwent modification, amorphous electron-transparent structures appeared, and tubular membrane structures were formed. No changes were found in the inactive variant. When Act. aureofaciens grows in conditions of non-regulated fermentation, it shows rapid disintegration and autolysis; then, wide fragmented hyphae are formed. The morphology of these hyphae is similar to that of spore forming hyphae of the aerial mycelium. Viable hyphae exist for a long time if the active culture grows in conditions of regulated fermentation with periodic addition of nutrient substances. Amorphous electron-transparent structures, apparently of the antibiotic nature, are formed in the hyphae and liberated into the medium.
对产四环素的金色链霉菌及其无活性的类原放线菌变体在调控发酵和非调控发酵条件下生长时进行了电子显微镜研究。活性变体的超微结构发生了变化：细胞壁和核糖体发生了改变，出现了无定形电子透明结构，并形成了管状膜结构。在无活性变体中未发现变化。当金色链霉菌在非调控发酵条件下生长时，它会迅速解体并自溶；然后，形成宽的断裂菌丝。这些菌丝的形态与气生菌丝的产孢菌丝相似。如果活性培养物在定期添加营养物质的调控发酵条件下生长，活菌丝会长期存在。菌丝中形成了明显具有抗生素性质的无定形电子透明结构，并释放到培养基中。
