Makarevich V G, Upiter G D, Slugina M D, Tarasova S S, Gravit N F
Antibiotiki. 1975 Apr;20(4):295-9.
A low initial rate of the culture growth is an obligatory condition for intensive biosynthesis of tetracycline by Act. aureofaciens in batch fermentations. The mineral phosphorus concentration is a factor limiting the growth rate. Under conditions of model experiments, the maximum specific rate of the culture growth equaled to 0.07--0.08 hours-1 was observed in the presence of at least 40 gamma/ml of phosphorus: such a rate correlated with the mycelium productivity equaled to 0.40--0.42 gamma/mg/hour. Cultures growing at a rate of 0.02--0.03 hours-1 were most productive (4.8--4.2 gamma/mg/hour). Such a rate was attained at the phosphorus concentration of 25--30 gamma/ml.
在分批发酵中，金黄色链霉菌大量生物合成四环素的一个必要条件是培养物生长的初始速率较低。矿物质磷浓度是限制生长速率的一个因素。在模型实验条件下，当磷浓度至少为40微克/毫升时，观察到培养物生长的最大比速率为0.07 - 0.08小时⁻¹ ：这样的速率与菌丝体生产力为0.40 - 0.42微克/毫克/小时相关。以0.02 - 0.03小时⁻¹ 的速率生长的培养物生产力最高（4.8 - 4.2微克/毫克/小时）。在磷浓度为25 - 30微克/毫升时可达到这样的速率。
