铜绿假单胞菌和荧光假单胞菌的D-葡萄糖酸脱氢酶以及产黑色素葡萄糖杆菌的2-酮-D-葡萄糖酸脱氢酶中共价结合黄素的鉴定。
Identification of the covalently bound flavins of D-gluconate dehydrogenases from Pseudomonas aeruginosa and Pseudomonas fluorescens and of 2-keto-D-gluconate dehydrogenase from Gluconobacter melanogenus.
作者信息
McIntire W, Singer T P, Ameyama M, Adachi O, Matsushita K, Shinagawa E
出版信息
Biochem J. 1985 Nov 1;231(3):651-4. doi: 10.1042/bj2310651.
Abstract
An improved method is presented for the purification of 8 alpha-(N1-histidyl)riboflavin, 8 alpha-(N3-histidyl)riboflavin and their 2',5'-anhydro forms, which permits the isolation of sizeable quantities of each of these compounds from a synthetic mixture in pure form. Flavin peptides were isolated from the D-gluconate dehydrogenases of Pseudomonas aeruginosa and Pseudomonas fluorescens and from the 2-keto-D-gluconate dehydrogenase of Gluconobacter melanogenus. After conversion into the aminoacyl-riboflavin, the flavin in all three enzymes was identified as 8 alpha-(N3-histidyl)riboflavin. By sequential treatment with nucleotide pyrophosphatase and alkaline phosphatase, the flavin in each enzyme was shown to be in the dinucleotide form.
摘要
本文提出了一种改进的方法用于纯化8α-(N1-组氨酰)核黄素、8α-(N3-组氨酰)核黄素及其2',5'-脱水形式，该方法能够从合成混合物中以纯形式分离出相当数量的上述每种化合物。从铜绿假单胞菌和荧光假单胞菌的D-葡萄糖酸脱氢酶以及产黑色素葡萄糖杆菌的2-酮-D-葡萄糖酸脱氢酶中分离出黄素肽。在转化为氨酰核黄素后，这三种酶中的黄素均被鉴定为8α-(N3-组氨酰)核黄素。通过依次用核苷酸焦磷酸酶和碱性磷酸酶处理，表明每种酶中的黄素均为二核苷酸形式。
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8 alpha-(O-Tyrosyl)flavin adenine dinucleotide, the prosthetic group of bacterial p-cresol methylhydroxylase.8α-（O-酪氨酰）黄素腺嘌呤二核苷酸，细菌对甲酚甲基羟化酶的辅基。
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D-Gluconate dehydrogenase from bacteria, 2-keto-D-gluconate-yielding, membrane-bound.来自细菌的D-葡萄糖酸脱氢酶，产生2-酮-D-葡萄糖酸，膜结合型。
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Characterization of methylamine dehydrogenase from bacterium W3A1. Interaction with reductants and amino-containing compounds.来自细菌W3A1的甲胺脱氢酶的特性。与还原剂和含氨基化合物的相互作用。
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Studies on succinate dehydrogenase. 8 -Histidyl-FAD as the active center of succinate dehydrogenase.琥珀酸脱氢酶的研究。8-组氨酰黄素腺嘌呤二核苷酸作为琥珀酸脱氢酶的活性中心。
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9
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Biochemistry. 1976 Jul 13;15(14):2937-45. doi: 10.1021/bi00659a001.
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