Shioda M, Higaki T, Mori K, Ikegami S
Biochem Biophys Res Commun. 1985 Nov 15;132(3):871-7. doi: 10.1016/0006-291x(85)91888-1.
Single-stranded circular DNA of phage fd was microinjected into Xenopus laevis eggs and DNA synthesis was analyzed after incubation. An efficient DNA synthesis occurred in the eggs: 2.7 ng of DNA was newly formed in an egg whose volume is approximately 900 nl during a 5-h incubation at 20 degrees C. The DNA synthesis was sensitive to aphidicolin, suggesting that DNA polymerase alpha participates in the synthesis. Agarose gel electrophoresis showed that full size of fd DNA is synthesized and that replicative form I of fd DNA is the major form of the newly produced DNAs. These results suggest that priming of DNA synthesis, elongation of DNA chain and ligation of DNA strands take place co-operatively in the egg with single-stranded DNA as a template.
将噬菌体fd的单链环状DNA显微注射到非洲爪蟾卵中，并在孵育后分析DNA合成情况。卵中发生了高效的DNA合成：在20℃下孵育5小时期间，体积约为900 nl的一个卵中新形成了2.7 ng DNA。DNA合成对阿非科林敏感，这表明DNA聚合酶α参与了合成过程。琼脂糖凝胶电泳显示合成了全长的fd DNA，并且fd DNA的复制型I是新产生DNA的主要形式。这些结果表明，以单链DNA为模板，DNA合成的引发、DNA链的延伸和DNA链的连接在卵中协同发生。
