Riedel H D, König H, Knippers R
Biochim Biophys Acta. 1984 Nov 22;783(2):158-65. doi: 10.1016/0167-4781(84)90008-3.
We use specific restriction fragments as defined primers for DNA synthesis on single-stranded circular phage fd DNA. These structures are relatively poor templates for a highly purified DNA polymerase alpha from Xenopus laevis eggs. However, DNA synthesis is stimulated about 5-fold by addition of ATP to the reaction mixture. We show that the deoxynucleotide polymers, synthesized in the presence of ATP, are significantly longer than those produced in the absence of ATP. We also show that this effect is due to a more tenacious binding of DNA polymerase alpha to DNA and conclude that ATP increases the processivity of the enzyme.
我们使用特定的限制性片段作为在单链环状噬菌体fd DNA上进行DNA合成的定义引物。这些结构对于来自非洲爪蟾卵的高度纯化的DNA聚合酶α而言是相对较差的模板。然而，通过向反应混合物中添加ATP，DNA合成被刺激了约5倍。我们表明，在ATP存在下合成的脱氧核苷酸聚合物明显长于在没有ATP的情况下产生的聚合物。我们还表明，这种效应是由于DNA聚合酶α与DNA的结合更紧密，并得出结论，ATP增加了该酶的持续合成能力。
