Ma Kunpeng, Zhuo Rongfeng, Pi Zhilong, Zhang Jiaqi, Peng Xin, Ye Xiangling, Ma Xiangyang, Zhang Ying
The First School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong 510515, China; Guangdong Key Lab of Orthopedic Technology and Implant Materials, General Hospital of Southern Theatre Command of PLA, Guangzhou, Guangdong 510010, China.
Graduate School, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, China.
Colloids Surf B Biointerfaces. 2025 Dec;256(Pt 1):114976. doi: 10.1016/j.colsurfb.2025.114976. Epub 2025 Jul 22.
Bone defects resulting from fractures, bone tumor resections, and non-unions are common clinical challenges. 3D-printed bone scaffolds offer enhanced customization and adaptability. however, their existing limitations in biological activity need to be addressed. In this study, we developed a novel composite scaffold (PLGA/CaSO4/PDA/ICA) for bone defect repair by incorporating polydopamine (PDA) and icariin (ICA) into a PLGA/CaSO4 matrix. We utilized Fused Deposition Modeling (FDM) technology to fabricate PLGA/CaSO scaffolds and employed polydopamine (PDA) for the effective loading of ICA. Characterization tests revealed that the PLGA/CaSO/PDA/ICA scaffolds exhibited excellent hydrophilicity and mechanical properties. In vitro experiments showed that the PLGA/CaSO/PDA/ICA scaffolds significantly promoted cell proliferation, migration, and osteogenic differentiation while enhancing the expression of osteogenesis-related genes. Additionally, in vivo experiments revealed accelerated bone regeneration in the PLGA/CaSO4/PDA/ICA group, displaying higher bone volume fraction (BV/TV), denser trabeculae, and deeper scaffold integration at 8 weeks. Overall, These findings highlight the synergistic role of PDA-mediated surface modification and ICA-driven osteoinduction, positioning the PLGA/CaSO4/PDA/ICA scaffold as a promising candidate for bone tissue engineering applications.
由骨折、骨肿瘤切除和骨不连导致的骨缺损是常见的临床挑战。3D打印骨支架具有更强的定制性和适应性。然而，它们在生物活性方面存在的局限性仍需解决。在本研究中，我们通过将聚多巴胺（PDA）和淫羊藿苷（ICA）加入PLGA/CaSO4基质中，开发出一种用于骨缺损修复的新型复合支架（PLGA/CaSO4/PDA/ICA）。我们利用熔融沉积建模（FDM）技术制造PLGA/CaSO支架，并使用聚多巴胺（PDA）有效负载ICA。表征测试显示，PLGA/CaSO/PDA/ICA支架具有优异的亲水性和机械性能。体外实验表明，PLGA/CaSO/PDA/ICA支架显著促进细胞增殖、迁移和成骨分化，同时增强成骨相关基因的表达。此外，体内实验显示PLGA/CaSO4/PDA/ICA组的骨再生加速，在8周时显示出更高的骨体积分数（BV/TV）、更密集的骨小梁和更深的支架整合。总体而言，这些发现突出了PDA介导的表面修饰和ICA驱动的骨诱导的协同作用，使PLGA/CaSO4/PDA/ICA支架成为骨组织工程应用中有前景的候选材料。
