植物磷脂转移蛋白的体外合成：高效液相色谱法研究
In vitro synthesis of a plant phospholipid transfer protein: a study by high performance liquid chromatography.
作者信息
Tchang F, Laroche-Raynal M, Vergnolle C, Demandre C, Douady D, Grosbois M, Guerbette F, Delseny M, Kader J C
出版信息
Biochem Biophys Res Commun. 1985 Nov 27;133(1):75-81. doi: 10.1016/0006-291x(85)91843-1.
In order to study the biosynthesis of a plant phospholipid transfer protein (PLTP), poly (A)+RNAs have been prepared from maize seedlings and translated in vitro with a rabbit reticulocyte lysate. The newly synthesized proteins were then separated by fast protein liquid chromatography (FPLC) followed by SDS-PAGE or by high performance liquid chromatography (HPLC) coupled to a radioactivity detector monitor. It has been showed that a radioactive band comigrating with a 14C methylated pure PLTP was detected by SDS-PAGE. This result, confirmed by direct radioactive monitoring, indicates that PLTP is actively synthesized in vitro.
为了研究植物磷脂转移蛋白（PLTP）的生物合成，已从玉米幼苗中制备了聚腺苷酸（poly (A)+）RNA，并在兔网织红细胞裂解物中进行体外翻译。然后通过快速蛋白质液相色谱（FPLC），接着进行SDS-PAGE，或通过与放射性检测器监测器联用的高效液相色谱（HPLC）分离新合成的蛋白质。通过SDS-PAGE检测到一条与14C甲基化的纯PLTP共迁移的放射性条带。经直接放射性监测证实，该结果表明PLTP在体外被积极合成。
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