将人类血细胞化学重编程为多能干细胞。

Chemical reprogramming of human blood cells to pluripotent stem cells.

作者信息

Peng Fangqi, Wang Yanglu, Cheng Lin, Cai Ruyi, Fu Xiaodi, Yang Zhihan, Cheng Ruoqi, Zeng Weizhen, Dong Yingshuai, Cao Jingxiao, Mao Jingping, Zeng Jingran, Liu Tianxing, Chen Guanxian, Lei Qi, Wang Lipeng, Liu Lulu, Sun Shicheng, Li Cheng, Mu Rong, Guan Jingyang, Deng Hongkui

机构信息

MOE Key Laboratory of Cell Proliferation and Differentiation, School of Life Sciences and MOE Engineering Research Center of Regenerative Medicine, School of Basic Medical Sciences, State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China.

State Key Laboratory of Natural and Biomimetic Drugs, Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Sciences, Peking University, Beijing, China.

出版信息

Cell Stem Cell. 2025 Aug 7;32(8):1192-1199.e11. doi: 10.1016/j.stem.2025.07.003. Epub 2025 Jul 30.

DOI:10.1016/j.stem.2025.07.003

PMID:40744016

Abstract

Chemical reprogramming offers a fundamentally innovative approach for generating human chemically induced pluripotent stem (hCiPS) cells using small molecules. Our recent studies showed that this approach was highly efficient in reprogramming human fibroblasts to hCiPS cells. However, generating hCiPS cells from human blood cells, which are the most accessible and convenient source for reprogramming, remains a challenge. In this study, we established a robust method that successfully generated hCiPS cells from both cord blood and adult peripheral blood cells. This method achieved efficient reprogramming with both fresh and cryopreserved blood cells across different donors. Notably, this method also efficiently generated an average of over 100 hCiPS colonies from just a single drop of fingerstick blood. These results highlight the advantages of chemical reprogramming for generating hCiPS cells from a blood source and represent a next-generation platform for efficient, scalable, and convenient stem cell production with broad applications in regenerative medicine.

摘要

化学重编程提供了一种使用小分子生成人类化学诱导多能干细胞（hCiPS细胞）的根本性创新方法。我们最近的研究表明，这种方法在将人类成纤维细胞重编程为hCiPS细胞方面非常高效。然而，从人类血细胞（这是重编程最容易获得且方便的来源）生成hCiPS细胞仍然是一项挑战。在本研究中，我们建立了一种可靠的方法，该方法成功地从脐带血和成人外周血细胞中生成了hCiPS细胞。这种方法在不同供体的新鲜和冷冻保存血细胞中均实现了高效重编程。值得注意的是，这种方法仅从一滴指尖血就能高效地平均产生超过100个hCiPS克隆。这些结果突出了化学重编程从血液来源生成hCiPS细胞的优势，并代表了一个用于高效、可扩展且方便的干细胞生产的下一代平台，在再生医学中有广泛应用。