Li Yinzheng, Guo Guixin, Chen Tianying, Lu Peng, Tang Yanjun
State Key Laboratory of Bio-based Fiber Materials, Zhejiang Sci-Tech University, Hangzhou 310018, PR China.
State Key Laboratory of Bio-based Fiber Materials, Zhejiang Sci-Tech University, Hangzhou 310018, PR China.
Int J Biol Macromol. 2025 Sep;321(Pt 3):146420. doi: 10.1016/j.ijbiomac.2025.146420. Epub 2025 Jul 29.
To promote the functionalization of chitosan-based films, lignin separated from Moso bamboo using type IV deep eutectic solvents (DESs; FeCl/glycerol (Gly), AlCl/Gly, and CuCl/Gly) was incorporated into chitosan to prepare lignin/chitosan composite films. For comparison, lignin pretreated with an aqueous NaOH solution was prepared and used for forming a film. The systematic characterization of lignin samples and lignin/chitosan composite films was performed using advanced analytical techniques. Gel permeation chromatography showed that the type-IV-DES-pretreated lignin sample possessed a lower molecular weight and more uniform structure than the NaOH-pretreated lignin sample, which was more conducive to increasing the compatibility between lignin and chitosan. P nuclear magnetic resonance spectroscopy and two-dimensional heteronuclear correlation results revealed that the excellent antioxidant activity of lignin following AlCl/Gly pretreatment was due to the high phenolic hydroxyl group content (1.16 mmol/g). Moreover, the free-radical scavenging capacity of the resulting DES-pretreated lignin/chitosan composite film reached 83.12 %, which was 3.4-fold higher than that of the pure chitosan film. In general, type-IV-DES-pretreated lignin was superior to NaOH-pretreated lignin in terms of enhancing the ultraviolet-shielding ability, antioxidant capacity, and antibacterial properties of the chitosan films. Notably, these films have great potential for use in food packaging applications.
为促进壳聚糖基薄膜的功能化，将使用IV型低共熔溶剂（DESs；FeCl/甘油（Gly）、AlCl/Gly和CuCl/Gly）从毛竹中分离出的木质素掺入壳聚糖中，制备木质素/壳聚糖复合薄膜。为作比较，制备了用NaOH水溶液预处理的木质素并用于成膜。使用先进的分析技术对木质素样品和木质素/壳聚糖复合薄膜进行了系统表征。凝胶渗透色谱表明，与NaOH预处理的木质素样品相比，IV型DES预处理的木质素样品具有更低的分子量和更均匀的结构，这更有利于提高木质素与壳聚糖之间的相容性。磷核磁共振光谱和二维异核相关结果表明，AlCl/Gly预处理后木质素具有优异的抗氧化活性是由于其高酚羟基含量（1.16 mmol/g）。此外，所得DES预处理的木质素/壳聚糖复合薄膜的自由基清除能力达到83.12%，比纯壳聚糖薄膜高3.4倍。总体而言，IV型DES预处理的木质素在增强壳聚糖薄膜的紫外线屏蔽能力、抗氧化能力和抗菌性能方面优于NaOH预处理的木质素。值得注意的是，这些薄膜在食品包装应用中具有巨大潜力。
