Amaechi Bennett Tochukwu, Tan Alexandria Isabella, Noureldin Amal Ahmed K, Kanthaiah Kannan, Holladay Emily, Obiefuna Amos Chinedu, Vijayaraghavan Mahalakshmi
Department of Comprehensive Dentistry, University of Texas Health San Antonio, San Antonio, TX, USA.
Department of Comprehensive Dentistry, University of Texas Health San Antonio, San Antonio, TX, USA.
J Dent. 2025 Oct;161:106006. doi: 10.1016/j.jdent.2025.106006. Epub 2025 Jul 29.
Previous studies has shown that hydroxyapatite (HAP) in oral care formulations could serve as a favorable fluoride alternative for caries management. The present study compared the efficacy of different hydroxyapatite-containing toothpastes in remineralizing early caries lesions.
Early enamel caries lesion were produced on one hundred and sixty bovine enamel blocks by 3-day demineralization using a multispies microbial caries model. Following this, the 160 lesion-bearing blockswere randomly assigned to eight treatment groups (20/group): Crest cavity protection (1100 ppm fluoride), ClinPro 5000 (5000 ppm fluoride), Fygg (20 % nanoXIM), Boka (nanoXIM; concentration not disclosed), Dr. Jen (10 % nanoHAP), Risewell Regular (10 % microHAP), Risewell PRO (5 % nanoXIM + microHAP), and Just-Ingredients Powder (15 % microHAP). The blocks were subjected to remineralization by daily pH-cycling consisting of a 2-minute acidic challenge, three 2-minute toothpaste treatments, and then storage in artificial saliva for the rest of the time for 14 days. Surface microhardness (SMH) of each lesion was measured before and after remineralization. Data was analyzed statistically (α = 0.05) by paired t-test (intragroup comparisons) and ANOVA/Tukey tests (intergroup comparison).
Every product achieved significant (p < .001) increase in SMH (remineralization). Tukey multiple comparison test indicated that Fygg (39.81 ± 24.07; 95 %CI 26.99-52.64) had a statistically significantly higher percentage remineralization than Boka (19.5 ± 10.56; 95 %CI 14.25-24.75, p < .01), Dr. Jen (21.68 ± 6.72; 95 %CI 18.45-24.92, p < .05), and Risewell Regular (21.80 ± 7.88; 95 %CI 18.11-25.49, p < .05), but did not differ significantly from the other products. There were no significant differences in the percentage remineralization among the rest of the toothpastes.
Within the limits of this in vitro study, there are differences in the ability of HAP toothpastes to enhance remineralization of early enamel caries lesions. It demonstrated that combining nanoXIM and nanoHAP in a toothpaste significantly enhanced the effectiveness of HAP to remineralize early caries lesion.
先前的研究表明，口腔护理配方中的羟基磷灰石（HAP）可作为龋齿管理中一种良好的氟替代物。本研究比较了不同含羟基磷灰石牙膏对早期龋损再矿化的疗效。
使用多菌种微生物龋模型，通过3天的脱矿作用在160个牛牙釉质块上产生早期釉质龋损。之后，将这160个带有龋损的牙釉质块随机分为8个治疗组（每组20个）：佳洁士防蛀健齿牙膏（含1100 ppm氟化物）、ClinPro 5000（含5000 ppm氟化物）、Fygg（含20%纳米XIM）、Boka（纳米XIM；浓度未披露）、珍博士牙膏（含10%纳米HAP）、皓齿健常规款（含10%微HAP）、皓齿健专业款（含5%纳米XIM + 微HAP）和纯成分粉末（含15%微HAP）。通过每日pH循环进行再矿化，包括2分钟的酸性挑战、三次2分钟的牙膏处理，然后在人工唾液中其余时间储存14天。在再矿化前后测量每个龋损的表面显微硬度（SMH）。数据通过配对t检验（组内比较）和方差分析/图基检验（组间比较）进行统计学分析（α = 0.05）。
每种产品的SMH（再矿化）均有显著（p <.001）增加。图基多重比较检验表明，Fygg（39.81 ± 24.07；95%置信区间26.99 - 52.64）的再矿化百分比在统计学上显著高于Boka（19.5 ± 10.56；95%置信区间14.25 - 24.75，p <.01）、珍博士牙膏（21.68 ± 6.72；95%置信区间18.45 - 24.92，p <.05）和皓齿健常规款（21.80 ± 7.88；95%置信区间18.
