David-Fung Elizabeth-Sharon, Belobrajdic Katherine A, Macke Jennifer P, Quackenbush Corey R, Ganguly Kumkum, Mamrosh Jennifer L
Theoretical Biology and Biophysics, Los Alamos National Laboratory, Los Alamos, NM, United States.
Front Immunol. 2025 Jul 17;16:1624020. doi: 10.3389/fimmu.2025.1624020. eCollection 2025.
Over the course of a few years, a small percentage of individuals with HIV-1 develop broadly neutralizing antibodies (bnAbs) capable of neutralizing diverse viruses. Although hundreds of antibodies with neutralizing activity against heterologous viruses have been referred to as bnAbs, there is no universally accepted numerical definition of a bnAb. Here, we will review important elements of HIV neutralizing antibodies and proposed definitions of bnAbs, as well as introduce a web-based tool, CAByN (Choose Antibodies by Neutralization), allowing users to identify antibodies meeting their numerical definitions of a bnAb from data in the Los Alamos HIV Databases CATNAP (Compile, Analyze and Tally NAb Panels) antibody neutralization database. Biological findings from use of CAByN are also presented here, including differential neutralizing activity for certain antibodies across viral clades, and identification of antibodies with suspected incomplete neutralization. Website address: http://hiv.lanl.gov/content/sequence/CABYN/CABYN.html.
在几年的时间里，一小部分感染人类免疫缺陷病毒1型（HIV-1）的个体产生了能够中和多种病毒的广泛中和抗体（bnAbs）。尽管数百种具有针对异源病毒中和活性的抗体被称为bnAbs，但目前尚无关于bnAbs的普遍接受的数值定义。在此，我们将回顾HIV中和抗体的重要要素以及bnAbs的提议定义，并介绍一个基于网络的工具CAByN（通过中和选择抗体），该工具允许用户从洛斯阿拉莫斯HIV数据库CATNAP（编译、分析和统计中和抗体组）抗体中和数据库的数据中识别符合其bnAbs数值定义的抗体。本文还展示了使用CAByN的生物学发现，包括某些抗体对不同病毒分支的差异中和活性，以及疑似不完全中和抗体的鉴定。网站地址：http://hiv.lanl.gov/content/sequence/CABYN/CABYN.html 。
