Yogendraiah Kavyasree Marabanahalli, Sadanandan Bindu, Kyathsandra Natraj Lokesh, Vijayalakshmi Vaniyamparambath, Shetty Kalidas
Department of Biotechnology, M S Ramaiah Institute of Technology, Bengaluru, Karnataka, India.
Department of Microbiological Sciences, North Dakota State University, Fargo, ND, United States.
Front Bioeng Biotechnol. 2025 Jul 17;13:1572694. doi: 10.3389/fbioe.2025.1572694. eCollection 2025.
Biofilm in the emerging pathogen and the most prevalent Non- infections is linked to fouling of medical devices and virulence. The growth conditions (temperature, media pH, incubation time, inoculum size, and shaker speed) for clinical cultures of were optimized on silicone elastomer material by Central composite design based on Response surface methodology. Six clinical cultures (C4, U873, U951, U1179, U1309 and U1360) and a standard culture (MTCC-184) were chosen for the study. Growth and biofilm were quantified for all the cultures by crystal violet (biofilm), MTT (cell viability), calcofluor white (cell mass), and wet and dry weight (cell mass) measurements. Among the isolates, U951 was found to fit the CCD model. The non-normal distribution and heteroscedasticity of the data favored the transformation via CCD-integrated Johnson model profiler for the prediction of the optimal growth conditions. For U951 isolate, biofilm formation was impacted by temperature and incubation time. A direct correlation was observed between biofilm formation and cell viability, with variations in the cell mass in all the cultures. This is the first of its kind study to advance an silicone elastomer-based high-throughput growth model of for various applications, including the screening of potential therapeutics.
新兴病原体以及最常见的非感染性疾病中的生物膜与医疗器械的污染和毒力有关。基于响应面法的中心复合设计在硅橡胶材料上优化了[病原体名称未给出]临床培养的生长条件（温度、培养基pH值、培养时间、接种量和摇床速度）。选择了六种临床培养物（C4、U873、U951、U1179、U1309和U1360）和一种标准培养物（MTCC - 184）进行研究。通过结晶紫（生物膜）、MTT（细胞活力）、荧光增白剂（细胞量）以及湿重和干重（细胞量）测量对所有培养物的生长和生物膜进行了定量。在分离株中，发现U951符合中心复合设计模型。数据的非正态分布和异方差性有利于通过中心复合设计集成的约翰逊模型剖析器进行变换，以预测最佳生长条件。对于U951分离株，生物膜形成受温度和培养时间的影响。观察到生物膜形成与细胞活力之间存在直接相关性，所有培养物中的细胞量都有变化。这是同类研究中首次推进基于硅橡胶的[病原体名称未给出]高通量生长模型，用于包括潜在治疗药物筛选在内的各种应用。
