对单个中国仓鼠细胞的圆二色性研究。

Circular dichroism studies on single Chinese hamster cells.

作者信息

Maestre M F, Salzman G C, Tobey R A, Bustamante C

出版信息

Biochemistry. 1985 Sep 10;24(19):5152-7. doi: 10.1021/bi00340a600.

Abstract

The circular dichroism (CD) and circular intensity differential scattering (CIDS) contributions to the CD of single Chinese hamster (CHO) cells have been measured as a function of the position in the cell cycle. The data are analyzed in three main spectral regions: (1) the region above 290 nm (scattering region), (2) the 250-290-nm regions (nucleic acid absorption region), and (3) the region below 240 nm (protein absorption region). The results show that CD/CIDS microspectrophotometry is a good indicator of the cell cycle phase. The results are consistent with the view that chromatin is organized in chiral superstructures which differentially scatter circularly polarized light. These structures appear highly specific and repeatable as the cell passes through its cycle.

摘要

已测量了圆二色性（CD）和圆强度差散射（CIDS）对单个中国仓鼠（CHO）细胞CD的贡献，并将其作为细胞周期中位置的函数。数据在三个主要光谱区域进行分析：（1）290nm以上区域（散射区域），（2）250 - 290nm区域（核酸吸收区域），以及（3）240nm以下区域（蛋白质吸收区域）。结果表明，CD/CIDS显微分光光度法是细胞周期阶段的良好指标。这些结果与染色质以手性超结构组织的观点一致，该超结构会以不同方式散射圆偏振光。随着细胞经历其周期，这些结构显得高度特异且可重复。