Wang Yingfang, Zhang Ruinan, Wang Pengfei, Zhang Wenlu, Li Zhongjie, Pang Xinyue, Huang Fangfang, Wang Sensen, Liu Xingnuo, Zhang He
Henan University of Science and Technology, School of Basic Medical Science and Forensic Medicine, Luoyang, Henan, China.
Henan University of Science and Technology, School of Hospital, Luoyang, Henan, China.
Front Cell Infect Microbiol. 2025 Jul 17;15:1565714. doi: 10.3389/fcimb.2025.1565714. eCollection 2025.
To investigate the mechanism of biofilm-mediated resistance to berberine in .
The resistance of berberine against was induced by 1/2 MIC (minimum inhibitory concentration). Biofilm formation was detected by crystal violet staining. The mRNA level was detected by RT-qPCR, and the gene was determined. the -overexpressed strain was constructed. We measured the MIC of berberine against , as well as biofilm formation and the expression of mRNA.
The MIC after berberine induction was more than 32 times than the MIC before induction. the biofilm was significantly increased at 24, 48 and 72 hours (<0.01) after berberine induction. In addition, the amount of biofilm production at 24, 48 and 72 hours was 1.3, 1.51 and 1.98 times after berberine induction than that before induction, respectively. The expression of gene was significantly increased (=0.016) after induction compared with that before induction. the MIC of overexpressed strain was about 5.8 times that before induction. The expression of gene was significantly increased (=0.016), which was 5.8 times higher than that before induction. The MIC of overexpressed strain was 100 μg/mL. Biofilm formation in csgD-overexpressed strain was 2.9 times higher than that of the control. The expression of biofilm-related genes, , and , was 45, 22.5 and 1628 times higher than that of the control, respectively.
Berberine might increase biofilm formation by inducing the expression of gene, which might result in drug resistance in .
研究生物膜介导的对小檗碱耐药的机制。
用1/2 MIC（最低抑菌浓度）诱导小檗碱对[具体菌名未给出]的耐药性。通过结晶紫染色检测生物膜形成。用RT-qPCR检测mRNA水平，并测定[具体基因未给出]基因。构建[具体基因未给出]过表达菌株。我们测定了小檗碱对[具体菌名未给出]的MIC，以及生物膜形成和mRNA的表达。
小檗碱诱导后的MIC比诱导前高出32倍以上。小檗碱诱导后24、48和72小时生物膜显著增加（<0.01）。此外，小檗碱诱导后24、48和72小时生物膜产生量分别是诱导前的1.3、1.51和1.98倍。诱导后[具体基因未给出]基因的表达与诱导前相比显著增加（=0.016）。过表达菌株的MIC约为诱导前的5.8倍。[具体基因未给出]基因的表达显著增加（=0.016），比诱导前高5.8倍。过表达菌株的MIC为100μg/mL。csgD过表达菌株中的生物膜形成比对照高2.9倍。生物膜相关基因[具体基因未给出]、[具体基因未给出]和[具体基因未给出]的表达分别比对照高45、22.5和1628倍。
小檗碱可能通过诱导[具体基因未给出]基因的表达增加生物膜形成，这可能导致[具体菌名未给出]产生耐药性。
