Chai Hua, Yang Li, Qu Xiaolin, Miao Peng
University of Science and Technology of China, Hefei 230026, China.
Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China.
JACS Au. 2025 Jun 21;5(7):3039-3044. doi: 10.1021/jacsau.5c00597. eCollection 2025 Jul 28.
MicroRNAs (miRNAs) have the potential to be applied as effective biomarkers for early diagnosis of cancers. Electrochemical techniques exhibit advantages such as high sensitivity and ease of miniaturization. However, electrode interface perturbations may hinder electrochemical responses. To address the limitation, three-dimensional DNA triangular pyramid frustum nanostructures are designed on the surface of the electrode to support miRNA (miR-21 as an example) recognition and following target recycling. Due to the combination of DNA nanostructures and enzyme-mediated signal amplification, the sensitivity and selectivity of this electrochemical biosensor are enhanced. It also performs satisfactorily in human samples, which meets clinical detection requirements. Overall, the strategy provides new possibilities for accurate and reproducible miRNA assays and shows great potential applications in early disease diagnosis.
微小RNA（miRNA）有潜力作为癌症早期诊断的有效生物标志物。电化学技术具有高灵敏度和易于小型化等优点。然而，电极界面干扰可能会阻碍电化学反应。为解决这一局限性，在电极表面设计了三维DNA三角台纳米结构，以支持miRNA（以miR-21为例）识别及后续的靶标循环。由于DNA纳米结构与酶介导的信号放大相结合，这种电化学生物传感器的灵敏度和选择性得到了提高。它在人体样本中也表现良好，满足临床检测要求。总体而言，该策略为准确且可重复的miRNA检测提供了新的可能性，并在疾病早期诊断中显示出巨大的潜在应用价值。
