Lumbar spondylolisthesis can lead to symptoms of mechanical back pain and radiculopathy. Surgical treatment aims to address dynamic instability and foraminal stenoses. While various alternatives exist to treat this clinical presentation, the single-position prone lateral interbody fusion has recently emerged as a popular technique. This operative video illustrates the treatment of a grade 1 L3-4 spondylolisthesis with a single-position prone lateral transpsoas interbody fusion with percutaneous instrumentation using the Mazor X Stealth robotic system. Detailed step-by-step techniques with operative pearls are provided to help surgeons incorporate the nuances of robotic navigation to the single-position prone lateral procedure. The video can be found here: https://stream.cadmore.media/r10.3171/2025.4.FOCVID24148.