Fauziah Nisa, Sari Ika Puspa, Faridah Lia, Afriandi Irvan, Adrizain Ryadi, Nugraha Naufal Fakhri, Kurniawan Agnes
Division of Parasitology, Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, Indonesia.
Postgraduate Program, Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java Indonesia.
Iran J Parasitol. 2025 Apr-Jun;20(2):289-298. doi: 10.18502/ijpa.v20i2.19048.
Stunting, resulting from chronic malnutrition, increases susceptibility to infections due to immature immunity. and may contribute to stunting. We aimed to determine the characteristics of intestinal protozoan infection among stunting children.
A cross-sectional study was conducted in 2020 among 280 stunted children in Bandung Regency, West Java, Indonesia. Faecal specimens were collected, with portions preserved separately in 10% formaldehyde and RNA Later solution. Of these, 230 met the examination criteria. Risk factors and demographic data were obtained through interviews. DNA was extracted, and intestinal protozoan infection were detected using PCR targeting the 18S SSU rRNA gene for and 16S-like RNA gene for .
The prevalence of and was 5.6% (13/230) and 55.6% (128/230), respectively. Multivariate analysis identified age ( 0.004; OR 0.327) and no availability of a septic tank ( 0.021; OR 4.881) were the significant risk factors for infection. For infection, significant risk factors included age ( 0.033; OR 0.722) and gender ( 0.047; OR 1.742).
Stunting and intestinal protozoan infection present a dual burden. and infections were prevalent among stunted children. Significant risk factors included age and septic tank unavailability for , while age and gender were associated with infection. Improved sanitation and targeted interventions are essential to reduce infection risks.
慢性营养不良导致的发育迟缓会因免疫不成熟而增加感染易感性，且感染可能会导致发育迟缓。我们旨在确定发育迟缓儿童肠道原生动物感染的特征。
2020年在印度尼西亚西爪哇省万隆县对280名发育迟缓儿童进行了一项横断面研究。收集粪便标本，部分分别保存在10%甲醛和RNA Later溶液中。其中230名符合检查标准。通过访谈获取危险因素和人口统计学数据。提取DNA，使用针对18S SSU rRNA基因的PCR检测肠道原生动物感染，针对另一种原生动物使用16S样RNA基因检测。
某两种原生动物的感染率分别为5.6%（13/230）和55.6%（128/230）。多变量分析确定年龄（P = 0.004；比值比0.327）和没有化粪池（P = 0.021；比值比4.881）是前一种原生动物感染的显著危险因素。对于后一种原生动物感染，显著危险因素包括年龄（P = 0.033；比值比0.722）和性别（P = 0.047；比值比1.742）。
发育迟缓和肠道原生动物感染呈现双重负担。这两种原生动物感染在发育迟缓儿童中普遍存在。显著危险因素包括年龄和没有化粪池与前一种原生动物感染有关，而年龄和性别与后一种原生动物感染有关。改善卫生条件和有针对性的干预措施对于降低感染风险至关重要。
