Fedorov A N, Al'zhanova A T, Ovchinnikov L P
Biokhimiia. 1985 Oct;50(10):1639-45.
Upon fractionation of a mitochondria-free extract of rabbit reticulocytes into a ribosome-free extract and mono- and polyribosomes the bulk of the aminoacyl-tRNA synthetase activity was found in the fraction of mono- and polyribosomes. All the fifteen aminoacyl-tRNA synthetases were revealed, although in somewhat different quantities, in both fractions of the mitochondria-free reticulocyte extract. Aminoacyl-tRNA synthetases of the ribosome-free extract are found in two forms: RNA-binding one, and, the one having no affinity for high molecular weight RNAs. Aminoacyl-tRNA synthetases dissociated from the complexes with polyribosomes exist only in the RNA-binding form. All aminoacyl-tRNA synthetases can be removed from such complexes by an addition of 16S rRNA of E. coli, poly(U) or tRNA of rabbit reticulocytes. This testifies to labile association of aminoacyl-tRNA synthetases with the RNA-component of polyribosomes as well as to a rather nonspecific character of their interaction. After EDTA-induced dissociation of polyribosomes, the aminoacyl-tRNA synthetase activity was detected in the complex with both ribosomal subunits.
将兔网织红细胞的无线粒体提取物分级分离成无核糖体提取物、单核糖体和多核糖体后，发现大部分氨酰 - tRNA合成酶活性存在于单核糖体和多核糖体部分。在无线粒体网织红细胞提取物的两个部分中均发现了所有15种氨酰 - tRNA合成酶，尽管数量略有不同。无核糖体提取物中的氨酰 - tRNA合成酶有两种形式：一种是与RNA结合的形式，另一种对高分子量RNA没有亲和力。从与多核糖体的复合物中解离出来的氨酰 - tRNA合成酶仅以RNA结合形式存在。通过添加大肠杆菌的16S rRNA、聚尿苷酸（poly(U)）或兔网织红细胞的tRNA，可以从这些复合物中去除所有氨酰 - tRNA合成酶。这证明了氨酰 - tRNA合成酶与多核糖体的RNA成分之间存在不稳定的结合，以及它们相互作用具有相当非特异性的特征。在乙二胺四乙酸（EDTA）诱导多核糖体解离后，在与两个核糖体亚基的复合物中检测到了氨酰 - tRNA合成酶活性。
