大鼠肝细胞游离体系中tRNA组分对多肽合成的抑制作用。
Inhibition of polypeptide synthesis by tRNA fractions in rat liver cell-free systems.
作者信息
Igarashi K, Kurosawa R, Hara K, Hirose S
出版信息
J Biochem. 1975 Jun;77(6):1325-33.
The mechanism of inhibition of polypeptide synthesis by the addition of a tRNA fraction in a rat liver cell-free system was studied. The inhibition was found to occur at the step of aminoacyl-tRNA binding to ribosomes, in which aminoacyl-tRNA's were mainly responsible for the inhibition. The addition of EF-1 decreased the inhibition by the tRNA fraction. The tRNA fraction inhibited polypeptide synthesis in a polysome-S100 system under conditions in which poly U- and poly A-dependent polypeptide syntheses were not inhibited. The possibility that the aminoacyl-tRNA inhibitory activity functions through improper binding to the ribosomes in the polysome-S100 system is discussed.
研究了在大鼠肝脏无细胞体系中添加tRNA组分对多肽合成的抑制机制。发现这种抑制发生在氨酰tRNA与核糖体结合的步骤，其中氨酰tRNA是抑制的主要原因。添加EF-1可降低tRNA组分的抑制作用。在聚尿苷酸和聚腺苷酸依赖性多肽合成未受抑制的条件下，tRNA组分在多核糖体-S100体系中抑制多肽合成。讨论了氨酰tRNA抑制活性通过在多核糖体-S100体系中与核糖体的不适当结合发挥作用的可能性。
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