Gunsalus G L, Musto N A, Bardin C W, Kunz H W, Gill T J
Biol Reprod. 1985 Dec;33(5):1057-63. doi: 10.1095/biolreprod33.5.1057.
Male rats homozygous for the growth and reproduction complex (grc), which is closely linked to the major histocompatibility complex, are sterile due to a uniform arrest of spermatogenesis after formation of primary spermatocytes. The present study was conducted to determine whether grc influenced the secretion of the Sertoli cell product androgen-binding protein (rABP) in two F2 hybrid crosses. One was an F2 hybrid of the R10 and ACP strains and the other was an F2 hybrid population derived from the R16 and BI strains. These crosses were chosen because they allowed examination of the effects of grc on rABP secretion when this gene complex was associated with two different major histocompatibility complex backgrounds. In both crosses the rABP content of testes and epididymides from rats homozygous for grc were markedly reduced whereas heterozygotes were normal. By contrast, the concentration of rABP in plasma was normal or near normal at all ages. These observations are compatible with the hypothesis that grc influences the secretion of rABP from the apical portion of Sertoli cells into the tubular lumen and its subsequent transport to the epididymis, but has little or no effect on rABP secretion from the base of Sertoli cells into the blood. In other studies we have shown that infertile animals that have received prenatal x-ray or that are heterozygous for the Hre gene have patterns of rABP secretion similar to that of grc/grc homozygotes. It is therefore possible that the abnormal rABP secretion into tubular lumen in these animals is secondary to germ cell depletion, which is a factor common to grc/grc, Hre/+, and x-ray-treated rats.
生长与生殖复合体（grc）与主要组织相容性复合体紧密连锁，纯合该复合体的雄性大鼠不育，原因是初级精母细胞形成后精子发生过程一致停滞。本研究旨在确定grc在两个F2杂交组合中是否影响支持细胞产物雄激素结合蛋白（rABP）的分泌。一个组合是R10和ACP品系的F2杂交后代，另一个组合是R16和BI品系衍生的F2杂交群体。选择这些杂交组合是因为当该基因复合体与两种不同的主要组织相容性复合体背景相关联时，能够研究grc对rABP分泌的影响。在两个杂交组合中，grc纯合大鼠睾丸和附睾中的rABP含量均显著降低，而异合子则正常。相比之下，血浆中rABP的浓度在所有年龄段均正常或接近正常。这些观察结果与以下假设相符：grc影响rABP从支持细胞顶端部分分泌到管腔中以及随后向附睾的转运，但对支持细胞基部向血液中分泌rABP几乎没有影响。在其他研究中我们已经表明，接受过产前X射线照射的不育动物或Hre基因杂合的不育动物，其rABP分泌模式与grc/grc纯合子相似。因此，这些动物管腔中异常的rABP分泌可能继发于生殖细胞耗竭，这是grc/grc、Hre/+和经X射线处理的大鼠共有的一个因素。
