Salmeen I, Zacmanidis P, Jesion G, Feldkamp L A
Biophys J. 1985 Nov;48(5):681-6. doi: 10.1016/S0006-3495(85)83825-X.
Translational movements of mitochondria in cultured rat liver cells were characterized quantitatively by using a video camera to detect and a video digitizer-computer system to analyze fluorescent images of mitochondria stained with rhodamine 123. The centroids of the images of individual mitochondria were determined at selected time intervals and the paths followed by the mitochondria were defined by the paths of the centroids. The predominant translation motion of the mitochondria satisfied the formal conditions of a Brownian random walk for a free particle, although in several cases there was a slow drift superimposed on the random motion. The apparent diffusion coefficients were approximately 5 X 10(-12) cm2 s-1, and the drift speeds approximately 2 X 10(-3) micron s-1.
通过使用摄像机进行检测，并利用视频数字化仪 - 计算机系统分析用罗丹明123染色的线粒体荧光图像，对培养的大鼠肝细胞中线粒体的平移运动进行了定量表征。在选定的时间间隔确定单个线粒体图像的质心，线粒体所遵循的路径由质心的路径定义。线粒体的主要平移运动满足自由粒子布朗随机游走的形式条件，尽管在某些情况下，随机运动上叠加有缓慢漂移。表观扩散系数约为5×10（-12）cm2 s-1，漂移速度约为2×10（-3）微米 s-1。
