England J M
Blood Cells. 1985;11(1):61-76.
Erythrocyte histograms should be analysed in accordance with the ICSH recommended protocol to determine: How many red cell populations are present and the proportion of the total red cells which are included in each population. The central tendency (mode, median & mean) and dispersion (size ratio, geometric standard deviation & coefficient of variation) for each population. The proportion of microcytic or macrocytic cells in each population. Examples are given to show how the patient's diagnosis is assisted by careful analysis of the histograms and the effect of treatment monitored by sequential testing. Histograms can be generated from blood films or from aperture-impedance and light-scatter volume measurements. Volume measurements on these systems are influenced by the red cell shape and internal refractive index. Mean cell hemoglobin concentration affects both shape in the aperture-impedance orifice and internal refractive index in light-scatter systems. There is poor agreement between volume histograms obtained on the two measuring systems and the histograms available on current automated instruments provide no more useful information than can be obtained from the blood film. Automated instruments need to produce histograms with fewer artefacts and the histograms should then be examined in accordance with the ICSH protocol. This approach should maximise the diagnostic value of the complete blood count.
红细胞直方图应按照国际血液学标准化委员会（ICSH）推荐的方案进行分析，以确定：存在多少个红细胞群体以及每个群体中红细胞占总红细胞的比例。每个群体的集中趋势（众数、中位数和均值）和离散度（大小比、几何标准差和变异系数）。每个群体中微细胞或巨细胞的比例。文中给出了一些例子，展示了通过仔细分析直方图如何辅助患者诊断以及通过连续检测监测治疗效果。直方图可从血涂片或孔径阻抗和光散射体积测量中生成。这些系统上的体积测量受红细胞形状和内部折射率的影响。平均细胞血红蛋白浓度会影响孔径阻抗孔中的形状以及光散射系统中的内部折射率。两种测量系统获得的体积直方图之间一致性较差，并且当前自动仪器上的直方图所提供的有用信息并不比从血涂片获得的更多。自动仪器需要生成伪像更少的直方图，然后应根据ICSH方案检查这些直方图。这种方法应能使全血细胞计数的诊断价值最大化。
