Li Yan, He Lu, Xue Zhiwei, Liang Yu, Jiang Jiaying, Wei Yiyan, Li Huisi, He Huimin, Li Rongjun
School of food and pharmaceutical engineering, Zhaoqing University, Zhaoqing, Guangdong Province, P.R. China.
Lushan Botanical Garden, Jiangxi Province and Chinese Academy of Science, Jiujiang, Jiangxi Province, P.R. China.
PLoS One. 2025 Aug 1;20(8):e0329548. doi: 10.1371/journal.pone.0329548. eCollection 2025.
Origanum vulgare L. (oregano) essential oil showed strong antimicrobial potential, effectively inhibiting the growth of various microorganisms. In our study, oregano essential oil inhibited the growth of Staphylococcus aureus with a minimum inhibitory concentration (MIC) of 3.13 µL/mL and Bacillus cereus at 6.25 µL/mL. Peronophythora litchii growth was completely suppressed by oregano essential oil at a concentration of 12.5 µL/mL over a period of 7 days. GC-MS analysis identified carvacrol as the dominant component of the oregano essential oil. Transcriptomic analysis revealed that treatment of P. litchii with oregano essential oil resulted in 9,710 differentially expressed genes (DEGs) compared to the control. GO analysis indicated that these DEGs were primarily associated with glutathione metabolic processes, cytosolic ribosome function, and ubiquitin-like protein peptidase activity. Additionally, KEGG pathway analysis showed significant enrichment in proteasome, ribosome, and oxidative phosphorylation pathways. These findings demonstrate the potent antimicrobial efficacy of oregano essential oil against both bacterial pathogens and P. litchii, highlighting its promise as a natural antimicrobial agent.
牛至（Origanum vulgare L.）精油具有很强的抗菌潜力，能有效抑制多种微生物的生长。在我们的研究中，牛至精油对金黄色葡萄球菌的最低抑菌浓度（MIC）为3.13 µL/mL，对蜡样芽孢杆菌的最低抑菌浓度为6.25 µL/mL。在7天的时间里，浓度为12.5 µL/mL的牛至精油完全抑制了荔枝霜霉疫霉（Peronophythora litchii）的生长。气相色谱-质谱联用（GC-MS）分析确定香芹酚是牛至精油的主要成分。转录组分析显示，与对照相比，用牛至精油处理荔枝霜霉疫霉产生了9710个差异表达基因（DEG）。基因本体（GO）分析表明，这些差异表达基因主要与谷胱甘肽代谢过程、胞质核糖体功能和类泛素蛋白肽酶活性有关。此外，京都基因与基因组百科全书（KEGG）通路分析显示，蛋白酶体、核糖体和氧化磷酸化通路有显著富集。这些发现证明了牛至精油对细菌病原体和荔枝霜霉疫霉都有强大的抗菌功效，凸显了其作为天然抗菌剂的前景。
