Mushayakarara E C, Mantsch H H
Can J Biochem Cell Biol. 1985 Oct;63(10):1071-6. doi: 10.1139/o85-133.
Thermotropic phase characteristics of the platelet-activating factor 1-O-octadecyl-2-acetoyl-sn-glycero-3-phosphocholine in excess water were studied by Fourier transform infrared spectroscopic techniques. Infrared bands characteristic of the interface and alkyl chain regions were monitored between -4 and 40 degrees C. Spectral parameters derived from these infrared bands demonstrate that in an aqueous environment this highly bioreactive lipid can assume three different structures: a micellar form at temperatures above 21 degrees C, a crystalline lamellar coagel form at temperatures below 12 degrees C, and a lamellar gel form that exists at temperatures between 12 and 21 degrees C. The three structures are separated by two phase transitions: a lower temperature (T1) phase transition at 12 degrees C, that involves a structural rearrangement in the region of the interface, and an upper temperature (Tu) phase transition at 21 degrees C, that involves the "conformational melting" of the alkyl chains.
采用傅里叶变换红外光谱技术研究了过量水中血小板活化因子1 - O - 十八烷基 - 2 - 乙酰基 - sn - 甘油 - 3 - 磷酸胆碱的热致相特性。在 - 4至40摄氏度之间监测界面和烷基链区域的特征红外波段。从这些红外波段得出的光谱参数表明，在水性环境中，这种具有高度生物活性的脂质可呈现三种不同结构：温度高于21摄氏度时为胶束形式，温度低于12摄氏度时为结晶层状凝聚凝胶形式，温度在12至21摄氏度之间时为层状凝胶形式。这三种结构由两个相变分隔：12摄氏度时的低温（T1）相变，涉及界面区域的结构重排；21摄氏度时的高温（Tu）相变，涉及烷基链的“构象熔化”。
