Merrick B A, Mansfield B K, Nikbakht P A, Selkirk J K
Cancer Lett. 1985 Nov;29(2):139-50. doi: 10.1016/0304-3835(85)90152-1.
Human mammary tumor T 47D cells were examined for their capacity to metabolize benzo[a]pyrene (BaP) to gain insight into potential metabolic pathways for BaP in human epithelial tissue. Confluent cultures metabolized 95% of 4 microM BaP after 24 h incubation. BaP metabolites were analyzed from culture medium since only residual amounts remained in cells. Tetraols/triols, dihydrodiols, quinones and phenols were either unconjugated or existed as sulfate conjugates. Glucuronide conjugation was minor. Remaining water-soluble (WS) metabolites could be extracted with butanol or removed from culture medium protein with methanol/water and suggestive evidence indicates these may be glutathione conjugates. A portion of BaP-WS metabolites were covalently bound to medium protein. This latter phenomenon is attributed to translocation of reactive BaP metabolites across cell membranes which could potentially occur in vivo during cellular processing of BaP.
对人乳腺肿瘤T 47D细胞代谢苯并[a]芘（BaP）的能力进行了检测，以深入了解BaP在人上皮组织中的潜在代谢途径。汇合培养物在孵育24小时后代谢了4 microM BaP的95%。由于细胞中仅残留少量BaP，因此从培养基中分析BaP代谢物。四醇/三醇、二氢二醇、醌和酚类要么未结合，要么以硫酸酯结合物的形式存在。葡萄糖醛酸结合较少。剩余的水溶性（WS）代谢物可用丁醇提取，或用甲醇/水从培养基蛋白中去除，提示性证据表明这些可能是谷胱甘肽结合物。一部分BaP-WS代谢物与培养基蛋白共价结合。后一种现象归因于活性BaP代谢物跨细胞膜的转运，这在BaP的细胞处理过程中可能在体内发生。
