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茶树类甜蛋白（TLP）基因家族分析表明，CsTLP8有助于茶树抗云纹叶枯病。

Analysis of thaumatin-like protein (TLP) gene family in tea plant revealed that CsTLP8 contributes to tea blister blight resistance.

作者信息

Mi Xiaozeng, Tang Mengsha, Yang Chun, Liang Sihui, Chen Yao, Qiao Dahe, Chen Zhengwu

机构信息

Guizhou Tea Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou, 550006, China.

Guizhou Key Laboratory of Molecular Breeding for Characteristic Horticultural Crops, Guiyang, Guizhou, 550006, China.

出版信息

BMC Plant Biol. 2025 Aug 2;25(1):1014. doi: 10.1186/s12870-025-06996-8.

DOI:10.1186/s12870-025-06996-8
PMID:40753186
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12317584/
Abstract

BACKGROUND

Thaumatin-like proteins (TLPs) belong to the PR-5 family of the pathogenesis-related (PR) proteins, and have been widely reported in plants for their important role in resisting pathogenic bacteria. However, people know very little about the function it plays in helping tea plants resist tea blister blight.

RESULTS

In the tea plant, a total of 43 CsTLPs were detected. According to the evolutionary tree analysis, these genes can be divided into 9 subfamilies. Through promoter analysis, a substantial quantity of elements associated with stress, hormone response, as well as growth and development were identified. The collinearity analysis indicates that the sequences of multiple CsTLPs genes have high homology in tea plants. The combination of transcriptome analysis and qRT-PCR demonstrated that the expression of CsTLP8, CsTLP23, CsTLP28 and CsTLP34 were significantly up-regulated after tea blister blight infection. In addition, we have verified the functionality of CsTLP8. CsTLP8 is localized in cytoplasm, and has β-1,3-glucanase activity. Transient overexpression of CsTLP8 in tobacco can significantly increase POD and CAT activities and decrease hydrogen peroxide content.

CONCLUSIONS

The results will help to better understand the role of CsTLPs gene family in tea blister blight, and provide theoretical support for improving disease resistance of tea trees and breeding new varieties resistant to disease.

摘要

背景

类甜蛋白（TLPs）属于病程相关（PR）蛋白中的PR-5家族，在植物抵抗病原菌方面发挥重要作用，已在植物中被广泛报道。然而，人们对其在帮助茶树抵御茶饼病中所起的作用知之甚少。

结果

在茶树中，共检测到43个CsTLPs。根据进化树分析，这些基因可分为9个亚家族。通过启动子分析，鉴定出大量与胁迫、激素应答以及生长发育相关的元件。共线性分析表明，多个CsTLPs基因序列在茶树中具有高度同源性。转录组分析和qRT-PCR相结合表明，茶饼病感染后CsTLP8、CsTLP23、CsTLP28和CsTLP34的表达显著上调。此外，我们验证了CsTLP8的功能。CsTLP8定位于细胞质中，具有β-1,3-葡聚糖酶活性。在烟草中瞬时过表达CsTLP8可显著提高POD和CAT活性，并降低过氧化氢含量。

结论

这些结果将有助于更好地了解CsTLPs基因家族在茶饼病中的作用，并为提高茶树抗病性和培育抗病新品种提供理论支持。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed5e/12317584/352cf8558b35/12870_2025_6996_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed5e/12317584/86409437e6fc/12870_2025_6996_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed5e/12317584/8569935c951e/12870_2025_6996_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed5e/12317584/717c8935d25a/12870_2025_6996_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed5e/12317584/148142e4606f/12870_2025_6996_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed5e/12317584/13fc6287ccfd/12870_2025_6996_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed5e/12317584/352cf8558b35/12870_2025_6996_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed5e/12317584/86409437e6fc/12870_2025_6996_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed5e/12317584/8569935c951e/12870_2025_6996_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed5e/12317584/717c8935d25a/12870_2025_6996_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed5e/12317584/148142e4606f/12870_2025_6996_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed5e/12317584/13fc6287ccfd/12870_2025_6996_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ed5e/12317584/352cf8558b35/12870_2025_6996_Fig6_HTML.jpg

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