Synaptotagmin-1 serves as a primary Zn sensor to mediate spontaneous neurotransmitter release under pathological conditions.

In the pre-synapse, vesicle fusion is a crucial process for neurotransmitter release in the nervous system. Under physiological conditions, synaptotagmin-1 (Syt1) locks synaptic vesicles in a priming state, allowing them to undergo synchronized neurotransmitter release upon Ca activation. Elevation of intracellular ions during diseases or injuries may lead to uncontrolled neurotransmitter release independent on Ca influx. However, its underlying molecular mechanism remains elusive. Here we show that elevation of the intracellular Zn concentration leads to the increased frequency of spontaneous neurotransmitter release in hippocampal neurons. In the reconstituted system with neuronal SNAREs and Syt1, Zn markedly enhances the fusion efficiency of liposomes via its binding to the interface between tandem C2 domains of Syt1. Moreover, Syt1 exhibits an elevated capacity to bind to anionic vesicles in the context of interaction with Zn, which leads to an augmentation in vesicles docking within the synaptic active zone. Finally, the mutation of the tentative Zn-binding site (Syt1_3M) results in a loss of activation function in spontaneous release by Zn, while disruption of the primary interface and the polybasic region show negligible impact on the modulatory action of Zn. Thus, these findings suggest that with Zn, Syt1 adopts an alternative regulatory mode to drive spontaneous neurotransmitter release.