Hongyuan Huang, Mengchi Chen, Yingying Liang, Xiaowen Zheng, Qiaojuan Huang, Jianghua Liu
The Second Affiliated Hospital of Guangxi Medical University, Nanning, 530000, Guangxi, China.
The Affiliated Tumor Hospital of Guangxi Medical University, Nanning, 530000, Guangxi, China.
Sci Rep. 2025 Aug 2;15(1):28221. doi: 10.1038/s41598-025-13448-8.
Mitochondria-related genes (MRGs) are considered screening MRGs as biomarkers for important therapeutic targets, but their role in acute respiratory distress syndrome (ARDS) remains unclear. This study aimed to identify MRGs-related biomarkers for ARDS. Intersection of 2030 MRGs with 343 differentially expressed genes (DEGs) between sepsis-non-ARDS and sepsis-ARDS group yielded 20 MRGs-related DEGs enriched in mitochondrion-related pathways. Monocytes, T cells, natural killer (NK) T cells, NK cells, and innate lymphoid cells (ILCs) were screened as candidate key cell types. SLC2A1 and IFI27 were defined as biomarkers; naive B cells, resting NK cells, and naive CD4 T cells, as differential immune cells; and monocytes, as the key cell type, subdivided into the CD14 and CD16 subtypes. The sepsis-ARDS group exhibited enhanced CD14 Mono and B cells compared with the sepsis-non-ARDS group and CD16 had a higher percentage and score in the G2/M and S phases than CD14. IFI27 and SLC2A1 were mainly expressed differently in the CD14 monocytes in ARDS, and their mRNA expression levels in peripheral blood of the sepsis patients was upregulated compared with those of the healthy controls. Thus, our findings demonstrate that SLC2A1 and IFI27 represent novel MRGs-related diagnostic biomarkers for ARDS, providing theoretical references for its treatment.
线粒体相关基因（MRGs）被视为筛选MRGs作为重要治疗靶点的生物标志物，但其在急性呼吸窘迫综合征（ARDS）中的作用仍不清楚。本研究旨在确定与ARDS相关的MRGs生物标志物。2030个MRGs与脓毒症非ARDS组和脓毒症ARDS组之间的343个差异表达基因（DEGs）的交集产生了20个与MRGs相关的DEGs，这些基因富集于线粒体相关途径。筛选出单核细胞、T细胞、自然杀伤（NK）T细胞、NK细胞和固有淋巴细胞（ILCs）作为候选关键细胞类型。将SLC2A1和IFI27定义为生物标志物；将幼稚B细胞、静息NK细胞和幼稚CD4 T细胞定义为差异免疫细胞；将单核细胞定义为关键细胞类型，并细分为CD14和CD16亚型。与脓毒症非ARDS组相比，脓毒症ARDS组的CD14单核细胞和B细胞增多，且CD16在G2/M期和S期的百分比和评分高于CD14。IFI27和SLC2A1在ARDS患者的CD14单核细胞中表达差异主要不同，脓毒症患者外周血中它们的mRNA表达水平与健康对照相比上调。因此，我们的研究结果表明，SLC2A1和IFI27代表了与ARDS相关的新型MRGs诊断生物标志物，为其治疗提供了理论参考。
