马铃薯（Solanum tuberosum L.）磷效率的表型变异与全基因组关联研究

Phenotypic variability and genome-wide association studies in potato (Solanum tuberosum L.) for phosphorus efficiency.

作者信息

Hazarika Mousumi, Dehmer Klaus J, Uptmoor Ralf, Kavka Mareike, Kirchgesser Julian, Harpke Doerte, Bachmann-Pfabe Silvia

机构信息

Satellite Collections North, Gross Luesewitz Potato Collections (GLKS), Genebank Department, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Parkweg 3a, Sanitz OT, Gross Luesewitz, 18190, Germany.

Agronomy, Faculty for Agricultural and Environmental Sciences, University of Rostock, Justus-von-Liebig-Weg 6, Rostock, 18059, Germany.

出版信息

BMC Plant Biol. 2025 Aug 2;25(1):1012. doi: 10.1186/s12870-025-07018-3.

DOI:10.1186/s12870-025-07018-3

PMID:40753410

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12317545/

Abstract

BACKGROUND

Potatoes require phosphorus (P) for growth, tuber production and starch quality, but their small and shallow root system limits the nutrient acquisition. This results in excessive use of mineral P fertilisers, leading to environmental and economic concerns due to resource depletion. Identifying potato genotypes with high P efficiency and understanding the underlying genes responsible are crucial for molecular breeding and crop improvement. The present study aims at exploring the phenotypic and genotypic variation among potato genotypes from Gross Luesewitz Potato Collections (GLKS) and identifying markers significantly associated with P efficiency.

RESULTS

Phenotypic characterisation of a diversity set of 200 potato accessions for their response to P deprivation in a pot experiment showed a significant variance regarding biomass production, root length, plants height and P efficiency between the genotypes, with moderate to high heritability for these traits. Shoot biomass decreased by 66% on average under low P, while the root biomass decreased by 36% on average. The extent of the reduction was genotype specific, with some genotypes exhibiting higher root biomass and longer root length under low P than in control conditions. Outstanding genotypes were identified such as GLKS 11,578 (Kristall) with a dense and shallow root system or GLKS 10,591 (Tiger) with a long and extensive root system. Genome-wide association analysis identified 27 unique significant marker-trait associations including 13 associated with biomass, 3 with plant height and 11 with P efficiency, with a majority of them related to P utilization efficiency (7) and shoot biomass (11).

CONCLUSION

Our research is one the firsts to present a genome-wide association study in potatoes for P efficiency. Our study highlighted significant phenotypic variation among the genotypes, while promising targets for improving P efficiency traits in potato through genomic selection and marker-assisted breeding.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1186/s12870-025-07018-3.

摘要

背景

马铃薯生长、块茎生产和淀粉品质都需要磷（P），但其根系小且浅，限制了养分获取。这导致矿物磷肥的过度使用，由于资源枯竭而引发环境和经济问题。鉴定高磷效率的马铃薯基因型并了解其潜在基因对于分子育种和作物改良至关重要。本研究旨在探索来自格罗斯 - 吕瑟维茨马铃薯种质库（GLKS）的马铃薯基因型之间的表型和基因型变异，并鉴定与磷效率显著相关的标记。

结果

在盆栽试验中，对200份马铃薯种质对缺磷反应的表型特征进行了研究，结果表明，各基因型在生物量生产、根长、株高和磷效率方面存在显著差异，这些性状的遗传力为中度到高度。低磷条件下，地上部生物量平均下降66%，而根部生物量平均下降36%。下降程度因基因型而异，一些基因型在低磷条件下的根生物量和根长高于对照条件。鉴定出了一些优秀的基因型，如根系密集且浅的GLKS 11578（Kristall）或根系长且广泛的GLKS 10591（Tiger）。全基因组关联分析确定了27个独特的显著标记 - 性状关联，其中13个与生物量相关，3个与株高相关，11个与磷效率相关，其中大多数与磷利用效率（7个）和地上部生物量（11个）相关。