Helmann Tyler C, Filiatrault Melanie J, Stodghill Paul V
Emerging Pests and Pathogens Research Unit, Agricultural Research Service, United States Department of Agriculture, Robert W. Holley Center for Agriculture and Health, Ithaca, NY, United States.
Plant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, NY, United States.
Front Microbiol. 2022 Jan 25;13:778927. doi: 10.3389/fmicb.2022.778927. eCollection 2022.
species are causal agents of soft rot diseases in many economically important crops, including soft rot disease of potato (). Using random barcode transposon-site sequencing (RB-TnSeq), we generated genome-wide mutant fitness profiles of 3937, ME23, and 67-19 isolates collected after passage through several and conditions. Though all three strains are pathogenic on potato, 3937 is a well-characterized model while strains ME23 and 67-19 are recent isolates. Strain ME23 specifically was identified as a representative strain from a 2014 outbreak on potato. This study generated comparable gene fitness measurements across ecologically relevant conditions for both model and non-model strains. Tubers from the potato cultivars "Atlantic," "Dark Red Norland," and "Upstate Abundance" provided highly similar conditions for bacterial growth. Using the homolog detection software PyParanoid, we matched fitness values for orthologous genes in the three bacterial strains. Direct comparison of fitness among the strains highlighted shared and variable traits important for growth. Bacterial growth in minimal medium required many metabolic traits that were also essential for competitive growth , such as amino acid, carbohydrate, and nucleotide biosynthesis. Growth in tubers specifically required the pectin degradation gene . Disruption in three putative DNA-binding proteins had strain-specific effects on competitive fitness in tubers. Though the Soft Rot can cause disease with little host specificity, it remains to be seen the extent to which strain-level variation impacts virulence.
这些物种是许多经济上重要作物软腐病的病原体，包括马铃薯软腐病（）。我们使用随机条形码转座子位点测序（RB-TnSeq），生成了在经过几种环境和条件传代后收集的3937、ME23和67-19分离株的全基因组突变体适应性图谱。尽管这三个菌株对马铃薯都有致病性，但3937是一个特征明确的模型菌株，而ME23和67-19菌株是最近分离得到的。菌株ME23特别被鉴定为2014年马铃薯疫情中的代表性菌株。本研究针对模型菌株和非模型菌株在生态相关条件下生成了可比的基因适应性测量结果。马铃薯品种“大西洋”、“深红色诺兰”和“北部丰度”的块茎为细菌生长提供了高度相似的条件。使用同源物检测软件PyParanoid，我们匹配了三种细菌菌株中直系同源基因的适应性值。菌株间适应性的直接比较突出了对生长重要的共同和可变特征。在基本培养基中细菌生长需要许多对竞争性生长也至关重要的代谢特征，如氨基酸、碳水化合物和核苷酸生物合成。在块茎中生长特别需要果胶降解基因。三种假定的DNA结合蛋白的破坏对块茎中的竞争性适应性有菌株特异性影响。尽管软腐病菌能在几乎没有宿主特异性的情况下引发疾病，但菌株水平的变异对毒力影响的程度还有待观察。
