Sepp Krisztián, Kiss Péter Sándor, László Anna, Valkusz Zsuzsanna, Radács Marianna, Gálfi Márta, Molnár Zsolt
1 Szegedi Tudományegyetem, Szent-Györgyi Albert Orvostudományi Kar, Belgyógyászati Klinika, Endokrinológiai és Anyagcsere Osztály Szeged Magyarország.
2 Szegedi Tudományegyetem, Juhász Gyula Pedagógusképző Kar, Alkalmazott Egészségtudományi és Környezeti Nevelés Intézet, Környezet-biológia és Környezeti Nevelési Tanszék Szeged Magyarország.
Orv Hetil. 2025 Aug 3;166(31):1209-1216. doi: 10.1556/650.2025.33353.
Introduction: By the 3rd millennium, the Earth as a living environment had been altered by environmental stresses. For 25 years, our research group has been thematically engaged in model studies of homeostatic disruptor effects. These have enabled thematic studies to follow the effects of chemical agents, e.g., the combined effects of chlorobenzenes (hexachlorobenzene : 1,2,4-trichlorobenzene 1 : 1), which have endocrine disrupting properties. Objective: We aimed to develop a test model system to investigate the effects of endocrine disruptors in a validated test system. Method: In the test system, male and female Wistar rats were used as model animals for in vivo studies. Subtoxicity was controlled by liver enzymes and body weight and physiological characterization. The exposure pathway was standardized. Then, the organism-level sub-toxic dose range and the time windows for acute and chronic monitoring of subtoxicity were defined. Plasma arginine vasopressin, and oxytocin levels were measured by RIA and LIA. Data were evaluated using SAS and ANOVA. Results: A stable in vivo control system had to be established to monitor the biological effects. The chlorobenzene exposures were standardized via gastrointestinal probe, in a final volume of 1.0 ml per day, at doses of 0.1 and 1.0 µg/kg body weight. The chlorobenzene potency model compounds were optimized during in vivo gastrointestinal exposure. Exposures were standardized: via gastrointestinal probe, 1 ml final volume per day, at doses of 0.1 and 1.0 µg/kg body weight. Experimental time intervals were 0–90 days. Discussion: In our work, we have succeeded in developing a test system to establish a validated method for monitoring chemical environmental exposures. We have also developed a standardised procedure for the determination of human toxicity potentials. Conclusion: A validated test system has been developed to test for persistent organic pollutants, which are nowadays a major health concern, making their testing of real relevance in clinical practice. Orv Hetil. 2025; 166(31): 1209–1216.
到第三个千年时，地球作为一个生存环境已因环境压力而发生改变。25年来，我们的研究团队一直致力于稳态干扰物效应的模型研究。这些研究使得能够进行专题研究，以追踪化学制剂的效应，例如具有内分泌干扰特性的氯苯（六氯苯：1,2,4-三氯苯1:1）的联合效应。目的：我们旨在开发一种测试模型系统，以便在经过验证的测试系统中研究内分泌干扰物的效应。方法：在该测试系统中，雄性和雌性Wistar大鼠被用作体内研究的模型动物。通过肝酶、体重和生理特征来控制亚毒性。暴露途径实现了标准化。然后，确定了生物体水平的亚毒性剂量范围以及亚毒性急性和慢性监测的时间窗。通过放射免疫分析（RIA）和发光免疫分析（LIA）测量血浆精氨酸加压素和催产素水平。使用SAS和方差分析（ANOVA）对数据进行评估。结果：必须建立一个稳定的体内对照系统来监测生物学效应。通过胃肠探针将氯苯暴露标准化，每天最终体积为1.0 ml，剂量为0.1和1.0μg/kg体重。氯苯效力模型化合物在体内胃肠暴露期间得到优化。暴露实现了标准化：通过胃肠探针，每天最终体积为1 ml，剂量为0.1和1.0μg/kg体重。实验时间间隔为0至90天。讨论：在我们的工作中，我们成功开发了一种测试系统，以建立一种经过验证的监测化学环境暴露的方法。我们还开发了一种用于确定人类毒性潜力的标准化程序。结论：已开发出一种经过验证的测试系统，用于检测持久性有机污染物，如今这些污染物是主要的健康问题，这使得它们的测试在临床实践中具有实际意义。《匈牙利医学周报》。2025年；166(31):1209–1216。
