剪接、交换、延伸：活细胞中天然蛋白质的精准编辑。
Splice, exchange, extend: precision editing of native proteins in live cells.
作者信息
Schiefelbein Kevin, David Yael
机构信息
Chemical Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
Chemical Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA; Tri-Institutional PhD Program in Chemical Biology, New York, NY, USA; Department of Pharmacology, Weill Cornell Medicine, New York, NY, USA.
出版信息
Trends Biotechnol. 2025 Aug 2. doi: 10.1016/j.tibtech.2025.07.021.
Abstract
Beyer et al. and Hua et al. developed platforms for precise chemical editing of proteins in living mammalian cells. These approaches enabled site-specific tagging at diverse protein sites without disrupting function. Demonstrated on several key proteins, this technology opens new possibilities for studying protein dynamics and functions in live cells.
摘要
拜尔等人以及华等人开发了用于对活的哺乳动物细胞中的蛋白质进行精确化学编辑的平台。这些方法能够在不破坏功能的情况下对多种蛋白质位点进行位点特异性标记。在几种关键蛋白质上得到了验证，这项技术为研究活细胞中的蛋白质动力学和功能开辟了新的可能性。
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