Nishiyama Masaki, Miki Yuichiro, Tanaka Hiroaki, Natsuki Seiji, Kuroda Kenji, Yoshii Mami, Tamura Tatsuro, Toyokawa Takahiro, Lee Shigeru, Maeda Kiyoshi
Department of Gastroenterological Surgery, Osaka Metropolitan University Graduate School of Medicine, Osaka, Japan.
Cancer Sci. 2025 Aug 3. doi: 10.1111/cas.70163.
Infiltration of resident memory T cells (TRMs) in the main tumor has been reported as a favorable prognostic factor. However, the role of TRMs in the lymph nodes (LNs) remains unclear. Thus, we examined the prognostic impact of TRMs infiltration within LNs of patients with gastric cancer (GC). Among 151 patients with metastasis to LN station No. 3, we classified them into two groups (CD103 and CD103) based on the number of CD103 T cells using immunohistochemical staining and analyzed the association between these groups and survival outcomes. We also examined the phenotype of CD8 CD103 T cells in the metastatic LNs using flow cytometry. Among patients with LN metastasis, metastasis to LN station No. 3 was significantly associated with a poor prognosis. There was a significant correlation between the number of CD8 CD103 T cells between the main lesion and the metastatic LNs. CD103 was associated with a favorable prognosis (5-year overall survival [OS], log-rank p = 0.001; 5-year recurrence free survival [RFS], log-rank p = 0.001). Among adjuvant chemotherapy cases, patients with CD103 exhibited significantly better OS and RFS than those with CD103 (OS, log-rank p < 0.001; RFS, log-rank p < 0.001). Flow cytometry revealed that PD-1 expression in CD8 CD103 T cells was higher in metastatic than in normal LNs. Among patients with CD103, those with high PD-1 expression exhibited significantly better OS than those with low PD-1 expression. In conclusion, the infiltration of TRMs into LNs is a critical prognostic factor in GC.
据报道，主要肿瘤中驻留记忆T细胞（TRMs）浸润是一个有利的预后因素。然而，TRMs在淋巴结（LNs）中的作用仍不清楚。因此，我们研究了胃癌（GC）患者淋巴结内TRMs浸润的预后影响。在151例转移至第3组淋巴结的患者中，我们使用免疫组织化学染色根据CD103 + T细胞数量将他们分为两组（CD103 +和CD103 -），并分析了这些组与生存结果之间的关联。我们还使用流式细胞术检测了转移性淋巴结中CD8 + CD103 + T细胞的表型。在有淋巴结转移的患者中，转移至第3组淋巴结与预后不良显著相关。主要病变和转移性淋巴结之间CD8 + CD103 + T细胞数量存在显著相关性。CD103 +与良好的预后相关（5年总生存率[OS]，对数秩检验p = 0.001；5年无复发生存率[RFS]，对数秩检验p = 0.001）。在辅助化疗病例中，CD103 +患者的OS和RFS显著优于CD103 -患者（OS，对数秩检验p < 0.001；RFS，对数秩检验p < 0.001）。流式细胞术显示，转移性淋巴结中CD8 + CD103 + T细胞中PD-1表达高于正常淋巴结。在CD103 +患者中，PD-1高表达者的OS显著优于低表达者。总之，TRMs浸润至淋巴结是GC的关键预后因素。
