Kulshrestha Anmol, Gupta Pratima
Department of Biotechnology, National Institute of Technology Raipur, Raipur, India.
Future Microbiol. 2025 Aug;20(12):779-791. doi: 10.1080/17460913.2025.2539011. Epub 2025 Aug 3.
Microbial biofilms, particularly polymicrobial ones formed by and , exhibit increased resistance to conventional treatment. This study investigates the inhibitory effect of 4-hydroxyphenylacetic acid on Secreted Aspartyl Protease 5, a key virulence factor involved in hyphal development and biofilm formation in , to investigate its therapeutic potential in inhibiting polymicrobial biofilms developed with .
MATERIALS & METHODS: Biofilm formation was studied using a Biofilm Infection Simulator System and polymethylmethacrylate coupons. Biofilms were analyzed under untreated and 4-HPA-treated conditions. SAP5 proteinase activity on BSA was quantified in treated & un-treated cultures.
4-HPA treatment significantly restricted hyphal growth, reducing attachment. The biofilm decreased by over 78% in mono-species and 81% in polymicrobial cultures. The inhibition of SAP5 protease activity was 79% in mono-species and 65% in mixed cultures.
4-HPA effectively reduces biofilm formation and SAP5 activity, highlighting its potential as a biofilm-specific therapeutic for polymicrobial infections.
微生物生物膜，尤其是由[具体微生物1]和[具体微生物2]形成的多微生物生物膜，对传统治疗表现出更高的抗性。本研究调查了4-羟基苯乙酸对分泌性天冬氨酸蛋白酶5的抑制作用，该酶是参与[具体真菌]菌丝发育和生物膜形成的关键毒力因子，以研究其在抑制由[具体微生物1]和[具体微生物2]形成的多微生物生物膜方面的治疗潜力。
使用生物膜感染模拟系统和聚甲基丙烯酸甲酯试片研究生物膜形成。在未处理和4-羟基苯乙酸处理条件下对生物膜进行分析。在处理和未处理的培养物中对牛血清白蛋白上的分泌性天冬氨酸蛋白酶5活性进行定量。
4-羟基苯乙酸处理显著限制了[具体真菌]的菌丝生长，减少了[具体真菌]的附着。在单菌种培养中生物膜减少了78%以上，在多微生物培养中减少了81%。在单菌种培养中分泌性天冬氨酸蛋白酶5活性的抑制率为79%，在混合培养中为65%。
4-羟基苯乙酸有效减少生物膜形成和分泌性天冬氨酸蛋白酶5活性，突出了其作为多微生物感染生物膜特异性治疗药物的潜力。
