Jones Liam, Hanrahan Niall, Salta Maria, Skovhus Torben Lund, Thomas Kathryn, Illson Timothy, Wharton Julian, Webb Jeremy
School of Biological Sciences, University of Southampton, Southampton, UK.
School of Chemistry, Faculty of Engineering and Physical Sciences, University of Southampton, Southampton, UK.
Npj Mater Degrad. 2025;9(1):97. doi: 10.1038/s41529-025-00628-0. Epub 2025 Jul 31.
Understanding biocide performance in mixed-species biofilms is critical to mitigating microbiologically influenced corrosion (MIC). In this study, a novel dual anaerobic biofilm reactor was used to evaluate glutaraldehyde efficacy under environmentally relevant conditions, using a complex microbial consortium from marine sediment. Despite biocide dosing, biofilms persisted and induced localized corrosion, indicating incomplete mitigation. Each biocide application led to an electronegative shift in and a reduction in concentration, suggesting partial suppression of microbial activity. Raman spectroscopy and profilometry revealed differences in corrosion product composition and pit morphology between biotic and abiotic systems. 16S rRNA sequencing showed enrichment of stress-tolerant genera, including and , consistent with increased chemical tolerance. These findings highlight the limitations of conventional biocide strategies and demonstrate the need for adaptive, community-informed treatment approaches. The dual-reactor model provides a robust platform for future MIC standardization efforts and mechanistic investigation of biofilm resilience under anoxic conditions.
了解混合物种生物膜中的杀生剂性能对于减轻微生物影响的腐蚀（MIC）至关重要。在本研究中，使用了一种新型双厌氧生物膜反应器，在与环境相关的条件下，利用来自海洋沉积物的复杂微生物群落评估戊二醛的功效。尽管投加了杀生剂，但生物膜仍然存在并引发局部腐蚀，表明缓解不完全。每次使用杀生剂都会导致电极电位发生负向偏移以及溶解氧浓度降低，这表明微生物活性受到部分抑制。拉曼光谱和表面轮廓测量揭示了生物系统和非生物系统之间腐蚀产物组成和点蚀形态的差异。16S rRNA测序显示耐胁迫菌属（包括芽孢杆菌属和嗜盐单胞菌属）富集，这与化学耐受性增加一致。这些发现突出了传统杀生剂策略的局限性，并表明需要采用适应性的、基于群落信息的处理方法。双反应器模型为未来的MIC标准化工作以及缺氧条件下生物膜恢复力的机理研究提供了一个强大的平台。
