Jones Liam M, Hanrahan Niall, Salta Maria, Lund Skovhus Torben, Thomas Kathryn, Illson Timothy, Wharton Julian, Webb Jeremy S
School of Biological Sciences, University of Southampton, Southampton, United Kingdom.
School of Chemistry, Faculty of Engineering and Physical Sciences, University of Southampton, Southampton, United Kingdom.
Appl Environ Microbiol. 2025 Jun 18;91(6):e0222124. doi: 10.1128/aem.02221-24. Epub 2025 Mar 4.
Oilfield systems are a multifaceted ecological niche, which consistently experiences microbiologically influenced corrosion. However, simulating the environmental conditions of an offshore system within the laboratory is notoriously difficult. A novel dual anaerobic biofilm reactor protocol allowed a complex mixed-species marine biofilm to be studied. Interestingly, electroactive corrosive bacteria and fermentative electroactive bacteria growth was supported within the biofilm microenvironment. Critically, the biotic condition exhibited pits with a greater average area, which is characteristic of microbiologically influenced corrosion. This research seeks to bridge the gap between experimental and real-world scenarios, ultimately enhancing the reliability of biofilm management strategies in the industry.
It is becoming more widely understood that any investigation of microbiologically influenced corrosion requires a multidisciplinary focus on multiple lines of evidence. Although there are numerous standards available to guide specific types of testing, there are none that focus on integrating biofilm testing. By developing a novel dual anaerobic reactor model to study biofilms, insights into the different abiotic and biotic corrosion mechanisms under relevant environmental conditions can be gained. Using multiple lines of evidence to gain a holistic understanding, more sustainable prevention and mitigation strategies can be designed. To our knowledge, this is the first time all these metrics have been combined in one unified approach. The overall aim of this paper was to explore recent advances in biofilm testing and corrosion research and provide recommendations for future standards being drafted. However, it is important to note that this article itself is not intending to serve as a standard.
油田系统是一个多方面的生态位，一直受到微生物影响的腐蚀。然而，在实验室中模拟近海系统的环境条件非常困难。一种新颖的双厌氧生物膜反应器方案使得能够研究复杂的混合物种海洋生物膜。有趣的是，生物膜微环境中支持电活性腐蚀细菌和发酵性电活性细菌的生长。至关重要的是，生物条件下出现的凹坑平均面积更大，这是微生物影响腐蚀的特征。本研究旨在弥合实验与实际场景之间的差距，最终提高该行业生物膜管理策略的可靠性。
人们越来越广泛地认识到，任何对微生物影响腐蚀的研究都需要多学科关注多条证据。虽然有许多标准可用于指导特定类型的测试，但没有一个标准专注于整合生物膜测试。通过开发一种新颖的双厌氧反应器模型来研究生物膜，可以深入了解相关环境条件下不同的非生物和生物腐蚀机制。利用多条证据获得全面的理解，可以设计出更可持续的预防和缓解策略。据我们所知，这是首次将所有这些指标以一种统一的方法结合起来。本文的总体目标是探索生物膜测试和腐蚀研究的最新进展，并为正在起草的未来标准提供建议。然而，需要注意的是，本文本身并不打算作为一项标准。
