Liu Pen-Ju, Yuan Peng, Liu Shui-Ping
Department of Neurology, Beijing Anzhen Hospital, Capital Medical University, Beijing, 100029, People's Republic of China.
J Inflamm Res. 2025 Jul 28;18:10051-10059. doi: 10.2147/JIR.S519729. eCollection 2025.
This study aimed to examine the relationship between peripheral blood immune cell distribution patterns and both disease severity and prognosis in patients with multiple sclerosis (MS).
We conducted a retrospective analysis of clinical data from MS patients admitted to the Neurology Department of our Hospital between March 2020 and April 2024. Data collected included gender, age (years), disease duration (months), clinical manifestations, and results from blood and cerebrospinal fluid (CSF) tests. Disease severity and prognosis were assessed using the Expanded Disability Status Scale (EDSS) and Multiple Sclerosis Functional Composite (MSFC). Spearman correlation and multivariate linear regression (adjusted for age, gender, disease duration, and MS subtype) were employed to evaluate associations.
Of the MS patients studied, 10.4% (n=7) exhibited abnormal CSF pressure. The mean peripheral blood leukocyte count was 6.69 ×10/L, with neutrophils predominating (64.32% of total leukocytes), followed by lymphocytes (25.54%). The Neutrophil-to-Lymphocyte Ratio (NLR) and Monocyte-to-Lymphocyte Ratio (MLR) were 3.63 ± 2.30 and 0.33 ± 0.18, respectively. Among lymphocytes, T cells were most abundant (70.37%), with CD4T cells comprising 40.89% and CD8T cells at 27.80%. EDSS scores showed positive correlations with monocyte percentage (=0.027) and MLR (<0.001). ZMSFC scores negatively correlated with monocyte percentage (=0.007) and positively with T lymphocyte percentage (=0.006). Multivariate regression confirmed MLR (β = 1.02, P < 0.001) and monocyte percentage (β = 0.24, P = 0.004) as independent predictors of EDSS severity.
In MS patients, peripheral blood immune cells are predominantly neutrophils and lymphocytes, with CD4T and CD8T lymphocytes being the most prevalent lymphocyte subtypes. The distribution of these immune cells correlates significantly with MS disease severity and prognosis. Our findings suggest that immune cell subsets and MLR may serve as valuable indicators for monitoring MS progression and outcomes.
本研究旨在探讨多发性硬化症（MS）患者外周血免疫细胞分布模式与疾病严重程度及预后之间的关系。
我们对2020年3月至2024年4月期间我院神经内科收治的MS患者的临床资料进行了回顾性分析。收集的数据包括性别、年龄（岁）、病程（月）、临床表现以及血液和脑脊液（CSF）检查结果。使用扩展残疾状态量表（EDSS）和多发性硬化症功能综合评分（MSFC）评估疾病严重程度和预后。采用Spearman相关性分析和多因素线性回归分析（校正年龄、性别、病程和MS亚型）来评估相关性。
在所研究的MS患者中，10.4%（n = 7）表现出脑脊液压力异常。外周血白细胞平均计数为6.69×10⁹/L，以中性粒细胞为主（占白细胞总数的64.32%），其次是淋巴细胞（25.54%）。中性粒细胞与淋巴细胞比值（NLR）和单核细胞与淋巴细胞比值（MLR）分别为3.63±2.30和0.33±0.18。在淋巴细胞中，T细胞最为丰富（70.37%），其中CD4⁺T细胞占40.89%，CD8⁺T细胞占27.80%。EDSS评分与单核细胞百分比（r = 0.027）和MLR（r < 0.001）呈正相关。MSFC评分与单核细胞百分比（r = 0.007）呈负相关，与T淋巴细胞百分比（r = 0.006）呈正相关。多因素回归分析证实MLR（β = 1.02，P < 0.001）和单核细胞百分比（β = 0.24，P = 0.004）是EDSS严重程度的独立预测因素。
在MS患者中，外周血免疫细胞以中性粒细胞和淋巴细胞为主，其中CD4⁺T和CD8⁺T淋巴细胞是最常见的淋巴细胞亚型。这些免疫细胞的分布与MS疾病严重程度和预后显著相关。我们的研究结果表明，免疫细胞亚群和MLR可能是监测MS进展和预后的有价值指标。
