Antonov A S, Nikiforova G P, Krivosheev O G, Isachenkov V A
Probl Endokrinol (Mosk). 1977 May-Jun;23(3):80-3.
The Heterologous hard phase radioimmunochemical method (RICM) of luteinizing hormone (LH) determination was tested in a number of experimental models for specificity. The results obtained demonstrated that a possible factor of cross reaction with the thyrotropic hormone (TTH) and the folliculo-stimulating hormone (FSH) was apparently insignificant in determination of LH in rat serum. This conclusion was based on the following. An immunosorbent used in RICM, prepared from the antisera of rabbits immunized with sheep LH, failed to interact with the FSH of rat hypophysis extracts. TTH-releasing hormone failed to alter the level of the immunoreactive LH in rat serum, whereas LH-releasing hormone increased 31-fold the LH concentration in 20 min. Besides, by means of RICM there was recorded a marked increase of the LH in the serum of rats after ovariectomy and a fall of the LH concentration in response to the administration of estradiol together with progesteron to such rats.
采用异源硬相放射免疫化学方法（RICM）测定促黄体生成素（LH），并在多个实验模型中检测其特异性。所得结果表明，在测定大鼠血清中的LH时，与促甲状腺激素（TTH）和促卵泡激素（FSH）发生交叉反应的可能因素显然微不足道。该结论基于以下几点。RICM中使用的免疫吸附剂由用绵羊LH免疫的兔抗体制备而成，未能与大鼠垂体提取物中的FSH发生相互作用。促甲状腺激素释放激素未能改变大鼠血清中免疫反应性LH的水平，而促黄体生成素释放激素在20分钟内使LH浓度增加了31倍。此外，通过RICM记录到，去卵巢大鼠血清中的LH显著增加，而给这些大鼠同时注射雌二醇和孕酮后，LH浓度下降。
