Nikiforova G P, Antonov A S, Krivosheev O G, Isachenkov V A
Probl Endokrinol (Mosk). 1977 Jan-Feb;23(1):74-9.
Possibilities of creation of a radioimmunochemical method (RICM) of determination of the luteinising hormone (LH) were studied. The method is based on the application in the binding system of a water-insoluble immunosorbent obtained by copolymerization of antisera with isobutyl ester of chlorocarbonic acid. The antisera were obtained by immunization of rabbits with a highly-purified LH isolated from sheep hypophyses. The method is rapid and precise, and is carried out in a single stage. Significant results of RICM in the LH determination can be obtained with the minimal two-hour incubation time. Using different amounts of the immunosorbent in the reaction mixture with the same indicator dose of I125-LH it is possible to vary the method sensitivity (from 0.05 to 5 ng/test).
研究了建立一种测定促黄体生成素（LH）的放射免疫化学方法（RICM）的可能性。该方法基于在结合系统中应用通过抗血清与氯甲酸异丁酯共聚获得的水不溶性免疫吸附剂。抗血清是通过用从羊垂体中分离出的高度纯化的LH免疫兔子而获得的。该方法快速、精确，且在单一阶段进行。在最短两小时的孵育时间下，RICM在LH测定中可获得显著结果。在反应混合物中使用不同量的免疫吸附剂以及相同剂量的I125-LH指示剂，可以改变方法的灵敏度（从0.05到5 ng/测试）。
