[Simple and rapid heterologous method of radioimmunochemical determination of luteinizing hormone in the blood serum of rats. 1. Basic principles of the method].

Possibilities of creation of a radioimmunochemical method (RICM) of determination of the luteinising hormone (LH) were studied. The method is based on the application in the binding system of a water-insoluble immunosorbent obtained by copolymerization of antisera with isobutyl ester of chlorocarbonic acid. The antisera were obtained by immunization of rabbits with a highly-purified LH isolated from sheep hypophyses. The method is rapid and precise, and is carried out in a single stage. Significant results of RICM in the LH determination can be obtained with the minimal two-hour incubation time. Using different amounts of the immunosorbent in the reaction mixture with the same indicator dose of I125-LH it is possible to vary the method sensitivity (from 0.05 to 5 ng/test).