Krieger Théo, Taillebot Virginie, Maurel-Pantel Aurélien, Camy Claire, Edorh Grégoire, Ollivier Matthieu, Pithioux Martine
Aix Marseille Univ, CNRS, ISM, 13009 Marseille, France.
Aix Marseille Univ, APHM, CNRS, ISM, Sainte-Marguerite Hospital, Institute for Locomotion, Department of Orthopaedics and Traumatology, 13009 Marseille, France.
Bone Rep. 2025 Jul 25;26:101859. doi: 10.1016/j.bonr.2025.101859. eCollection 2025 Sep.
Bone allografts are frequently used in many surgical procedures because of their osteoconductive and osteoinductive properties. After being extracted from the donor, the graft is treated with a process that cleans and sterilizes it before being implanted in the patient. While they guarantee the safety of the patient receiving the graft, preservation processes often affect bone properties. This study aims to measure the effect of a supercritical CO process on the microarchitecture and the mechanical properties of trabecular bone at a microscopic scale using microindentation. 7 femoral heads were harvested from patients who had undergone a total hip arthroplasty. 42 cubic samples of 10 mm side from these femoral heads were randomly distributed in 3 groups: frozen at -20 °C, gamma irradiated and frozen at -20 °C, and treated with a supercritical CO process including gamma irradiation. All samples were imaged by microtomography and characterized by microindentation to correlate the bone microarchitecture with the mechanical properties at a microscopic scale. Our results show that the supercritical CO process exerts no significant effect on the microarchitecture parameters, indentation elastic modulus, and indentation hardness. The correlations between the microarchitecture and the mechanical properties revealed that gamma irradiation appears to induce a slight alteration in mechanical properties. However, the process combining a supercritical CO treatment and gamma irradiation does not induce any more alterations to the material than gamma irradiation itself. Thus, the supercritical CO process has no more impact than gamma irradiation on the mechanical properties of trabecular bone at the microscopic scale.
由于具有骨传导性和骨诱导性，骨移植常用于许多外科手术中。从供体取出后，移植物要经过清洁和消毒处理，然后再植入患者体内。虽然它们能保证接受移植物患者的安全，但保存过程往往会影响骨的特性。本研究旨在使用微压痕技术在微观尺度上测量超临界CO₂处理对小梁骨微观结构和力学性能的影响。从接受全髋关节置换术的患者身上采集了7个股骨头。从这些股骨头中切取边长为10毫米的42个立方体样本，随机分为3组：在-20°C下冷冻、γ射线辐照并在-20°C下冷冻、以及经过包括γ射线辐照的超临界CO₂处理。所有样本均通过显微断层扫描成像，并通过微压痕进行表征，以在微观尺度上关联骨微观结构和力学性能。我们的结果表明，超临界CO₂处理对微观结构参数、压痕弹性模量和压痕硬度没有显著影响。微观结构与力学性能之间的相关性表明，γ射线辐照似乎会导致力学性能略有改变。然而，与γ射线辐照本身相比，超临界CO₂处理和γ射线辐照相结合的过程对材料并没有造成更多的改变。因此，在微观尺度上，超临界CO₂处理对小梁骨力学性能的影响并不比γ射线辐照更大。
