Krieger Théo, Taillebot Virginie, Maurel-Pantel Aurélien, Lallemand Marylène, Edorh Grégoire, Ollivier Matthieu, Pithioux Martine
Aix Marseille Univ, CNRS, ISM, 13009, Marseille, France; Aix Marseille Univ, APHM, CNRS, ISM, Sainte-Marguerite Hospital, Institute for Locomotion, Department of Orthopaedics and Traumatology, 13009, Marseille, France; BIOBank®, Tissue Bank, 77127, Lieusaint, France; Ecole Centrale Marseille, 13013, Marseille, France.
Aix Marseille Univ, CNRS, ISM, 13009, Marseille, France; Aix Marseille Univ, APHM, CNRS, ISM, Sainte-Marguerite Hospital, Institute for Locomotion, Department of Orthopaedics and Traumatology, 13009, Marseille, France.
J Mech Behav Biomed Mater. 2025 Mar;163:106893. doi: 10.1016/j.jmbbm.2025.106893. Epub 2025 Jan 16.
Surgeons frequently use allograft bone due to its osteoconductive, osteoinductive, and osteogenic properties. Preservation processes are employed to clean the allograft, improve its conservation, and ensure its sterilization. Many current processes use the properties of supercritical CO to remove bone marrow. This study aims to measure the effect of a supercritical CO process on the microarchitecture and the mechanical properties of trabecular bone. Eleven femoral heads were harvested from patients undergoing total hip arthroplasty. Sixty-seven cubic samples with 10 mm sides from these femoral heads were distributed in 3 groups: frozen at -20 °C, gamma irradiated and frozen at -20 °C, and treated with a supercritical CO process including gamma irradiation. All the samples were tested with a microcomputer tomography scanner and a compression testing machine. The supercritical CO process has no significant effect on the microarchitectural parameters (BV/TV, Tb.th, Tb.sp, Tb.N, DA, and Conn.D). It has also no significant effect on the elastic modulus, yield stress, and ultimate stress. However, it has a significant effect on the densification stress. An advanced study on the correlation between the microarchitecture and the mechanical properties shows that for a given volume fraction of 0.26 (the mean value for our study), the elastic modulus and ultimate stress of the bone treated with supercritical CO were lower than those from the frozen group by 19% and 24% respectively.
由于同种异体骨具有骨传导性、骨诱导性和成骨特性，外科医生经常使用它。采用保存工艺来清洁同种异体骨，改善其保存状况，并确保其灭菌。目前许多工艺利用超临界CO₂的特性来去除骨髓。本研究旨在测量超临界CO₂处理对松质骨微观结构和力学性能的影响。从接受全髋关节置换术的患者身上采集了11个股骨头。从这些股骨头中获取了67个边长为10毫米的立方体样本，并分为3组：在-20°C下冷冻、γ射线辐照后在-20°C下冷冻，以及经过包括γ射线辐照在内的超临界CO₂处理。所有样本均使用微型计算机断层扫描仪和压缩试验机进行测试。超临界CO₂处理对微观结构参数（骨体积分数、骨小梁厚度、骨小梁间距、骨小梁数量、骨小梁离散度和骨连接密度）没有显著影响。它对弹性模量、屈服应力和极限应力也没有显著影响。然而，它对致密化应力有显著影响。一项关于微观结构与力学性能之间相关性 的深入研究表明，对于给定的体积分数0.26（我们研究的平均值），经超临界CO₂处理的骨的弹性模量和极限应力分别比冷冻组低19%和24%。
