de Matos Rafael Christian, Bitencourt Ana Flávia Alvarenga, de Oliveira Alexsandro Davi Mantovani, Moreira Carolina Paula de Souza, Machado Renes Resende, Scopel Marina
Faculdade de Farmácia, Universidade Federal de Minas Gerais, Avenida Antônio Carlos 6627, Campus Pampulha, 31.270-901 Belo Horizonte, MG, Brazil.
Centro Especializado Em Plantas Aromáticas, Medicinais e Tóxicas - CEPLAMT-Museu de História Natural e Jardim Botânico da Universidade Federal de Minas Gerais, Rua Gustavo da Silveira 1035, Horto, 31.080-010 Belo Horizonte, MG, Brazil.
ACS Omega. 2025 Jul 14;10(29):32003-32014. doi: 10.1021/acsomega.5c03580. eCollection 2025 Jul 29.
Plant biodiversity of Brazil offers significant potential for the development of phytotherapeutics, highlighting the spp. genus. Despite traditional use and ethnopharmacological support, species remain underexplored in scientific literature. This study aims to explore the flavonoids class, by optimizing flavonoid extraction from (Aubl.) leaves through response surface methodology and by developing and validating a high-performance liquid chromatography (HPLC) method. Parameters reported in the literature as important for the extraction of this genus were optimized using parametric statistical analysis. Extraction optimization was achieved evaluating key parameters, including extraction time (5,10 and 15 min), drug-to-solvent ratio (1:5, 1:10 and 1:15), temperature (20, 30 and 40 °C), and the number of extraction cycles (1, 2 and 3). The developed HPLC method was validated using myricitrin as the primary analytical marker and quercetin was chosen as external standard for quantitative purposes. Temperature was found to play a pivotal role in metabolite extraction from the plant raw material and the optimized condition consisted of two extraction cycles, each lasting 8 min, at 21 °C, with a drug-to-solvent ratio of 1:8. The optimized extract (10 mg/mL) was analyzed by HPLC using methanol as the diluent, with quercetin (29.5 μg/mL) as an external marker. The myricitrin content in the plant was determined to be 2.96 mg/g (0.30%). This study provides the first detailed characterization of a chemical marker for the species using an optimized extraction process that can be useful for other species of the genus in the future.
巴西的植物生物多样性为植物疗法的发展提供了巨大潜力，突出了 属植物。尽管有传统用途和民族药理学支持，但该物种在科学文献中仍未得到充分研究。本研究旨在通过响应面法优化从（奥布尔）叶中提取黄酮类化合物，并开发和验证一种高效液相色谱（HPLC）方法，从而探索黄酮类化合物类别。使用参数统计分析对文献中报道的对该属植物提取重要的参数进行了优化。通过评估关键参数实现了提取优化，这些参数包括提取时间（5、10和15分钟）、药-溶剂比（1:5、1:10和1:15）、温度（20、30和40°C）以及提取循环次数（1、2和3）。所开发的HPLC方法以杨梅素作为主要分析标记物进行验证，并选择槲皮素作为定量的外标物。发现温度在从植物原料中提取代谢物方面起着关键作用，优化条件包括两个提取循环，每个循环持续8分钟，温度为21°C，药-溶剂比为1:8。使用甲醇作为稀释剂，以槲皮素（29.5μg/mL）作为外标物，通过HPLC对优化提取物（10mg/mL）进行分析。测定该植物中杨梅素的含量为2.96mg/g（0.30%）。本研究首次使用优化提取工艺对该物种的化学标记物进行了详细表征，这在未来可能对该属的其他物种有用。
